Zymography

Methods and Protocols

  • Jeff Wilkesman
  • Liliana Kurz

Part of the Methods in Molecular Biology book series (MIMB, volume 1626)

Table of contents

  1. Front Matter
    Pages i-xx
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Jeff Wilkesman, Liliana Kurz
      Pages 3-10
  3. Endopeptidase Zymography

    1. Front Matter
      Pages 11-11
    2. Jeff Wilkesman
      Pages 25-31
    3. Handunge Kumudu Irani Perera
      Pages 43-52
    4. Péter Bencsik, Monika Bartekova, Anikó Görbe, Krisztina Kiss, János Pálóczi, Jana Radosinska et al.
      Pages 53-70
    5. Goran Mucić, Brankica Rašković, Natalija Polović
      Pages 71-78
    6. Zongli Ren, Juanjuan Chen, Raouf A. Khalil
      Pages 79-102
    7. Madathiparambil G. Madanan, Ambili Mechoor
      Pages 103-114
  4. Reverse Zymography and In Situ Zymography

    1. Front Matter
      Pages 123-123
    2. Kanika Sharma, Debasish Bhattacharyya
      Pages 125-132
    3. Aastha Chhabra, Vibha Rani
      Pages 133-143
  5. 2D Zymography

    1. Front Matter
      Pages 145-145
    2. Shanyan Chen, Fanjun Meng, Zhenzhou Chen, Zhe Qu, Jiankun Cui, Zezong Gu
      Pages 147-155
    3. Jeff Wilkesman, María Fernanda Padrón, Liliana Kurz, Hervé Rémignon
      Pages 157-166
  6. Special Zymography Cases

    1. Front Matter
      Pages 167-167

About this book

Introduction

This detailed volume examines the latest techniques and protocols associated with zymography, the practice of detecting enzyme activity on electrophoretic gels. Sections cover subjects such as endopeptidase zymography, reverse zymography & in situ zymography, 2D zymography, as well as a variety of special cases. Written in the highly respected Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and practical, Zymography: Methods and Protocols serves as an ideal aid for a broad array of researchers from the fields of biological and medicinal science, as well as scientists working in clinical and diagnostic medicine, medical genetics, agricultural entomology, genetic monitoring of environmental pollution, and forensic science.

Keywords

Enzyme activity Electrophoretic gels Endopeptidase zymography In situ zymography Isozymes and allozymes Enzymology Gene markers

Editors and affiliations

  • Jeff Wilkesman
    • 1
  • Liliana Kurz
    • 2
  1. 1.Centre for Environmental, Biology and Chemistry Research, Facultad de Ciencias y TecnologíaUniversity of CaraboboValenciaVenezuela
  2. 2.Centre for Environmental, Biology and Chemistry Research Facultad de Ciencias y TecnologíaUniversity of CaraboboValenciaVenezuela

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7111-4
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7109-1
  • Online ISBN 978-1-4939-7111-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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