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RNAi and Small Regulatory RNAs in Stem Cells

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Baohong Zhang

    1. Department of Biology, East Carolina University, Greenville, USA

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  • Includes cutting-edge methods and protocols for the study of the intersection of RNAi, small regulatory RNAs, and stem cell biology
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1622)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  3. RNA Interference

    1. Front Matter

      Pages 27-27
      Download chapter PDF

    2. Forward RNAi Screens in Human Hematopoietic Stem Cells

      • Roman Galeev, Christine Karlsson, Aurélie Baudet, Jonas Larsson
      Pages 29-50

    3. Silencing of ATP11B by RNAi-Induced Changes in Neural Stem Cell Morphology

      • Jiao Wang, Qian Wang, Fangfang Zhou, Dong Wang, Tieqiao Wen
      Pages 51-61

    4. High-Efficiency Transfection of Glioblastoma Cells and a Simple Spheroid Migration Assay

      • Carsten Hagemann, Diana Amend, Almuth F. Kessler, Thomas Linsenmann, Ralf-Ingo Ernestus, Mario Löhr
      Pages 63-79

    5. Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in Embryonic Stem Cells

      • Xiaoxing Cheng, Rui Jian
      Pages 81-89

    6. Efficient Depletion of Essential Gene Products for Loss-of-Function Studies in Embryonic Stem Cells

      • Soizik Berlivet, Isabelle Hmitou, Hélène Picaud, Matthieu Gérard
      Pages 91-100

    7. Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAi Directed Against Transcription Factor L3/Lhx8

      • Takayuki Manabe, Akio Wanaka
      Pages 101-109

    8. A Recessive Genetic Screen for Components of the RNA Interference Pathway Performed in Mouse Embryonic Stem Cells

      • Melanie I. Trombly, Xiaozhong Wang
      Pages 111-129

    9. RNAi Knockdown of Ape1 Gene in the Differentiation of Mouse Embryonic Stem Cells

      • Gang-Ming Zou, Jieqing Yu, Cynthia LeBron, Yumei Fu
      Pages 131-138

    10. An Efficient Transfection Method for Differentiation and Cell Proliferation of Mouse Embryonic Stem Cells

      • Jun-Yang Liou, Bor-Sheng Ko, Tzu-Ching Chang
      Pages 139-147

    11. Implanting Glioblastoma Spheroids into Rat Brains and Monitoring Tumor Growth by MRI Volumetry

      • Mario Löhr, Thomas Linsenmann, Anna Jawork, Almuth F. Kessler, Nils Timmermann, György A. Homola et al.
      Pages 149-159

    12. RNAi-Based Techniques for the Analysis of Gene Function in Drosophila Germline Stem Cells

      • Amelia J. Blake, Danielle S. Finger, Victoria L. Hardy, Elizabeth T. Ables
      Pages 161-184

    13. In vivo RNAi in the Drosophila Follicular Epithelium: Analysis of Stem Cell Maintenance, Proliferation, and Differentiation

      • Veit Riechmann
      Pages 185-206

    14. A Phenotype-Based RNAi Screening for Ras-ERK/MAPK Signaling-Associated Stem Cell Regulators in C. elegans

      • Myon-Hee Lee, Dong Suk Yoon
      Pages 207-221

  4. MicroRNAs

    1. Front Matter

      Pages 223-223
      Download chapter PDF

    2. Engineering Human Mesenchymal Stem Cells to Release Adenosine Using miRNA Technology

      • Gaoying Ren, Detlev Boison
      Pages 225-239
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Keywords

About this book

This detailed collection provides an accessible compendium of up-to-date methods focused on the study of RNAi and small regulatory miRNAs in stem cells. Beginning with a brief introductory section, the volume continues by exploring methods and protocols for RNAi screening, transfection, and the knockdown of specific genes and pathways in several animal species, including humans and mice, recently developed methods for miRNA expression and functional analysis, as well as usage of CRISPR/Cas 9 to knockout an individual gene for functional studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Practical and authoritative, RNAi and Small Regulatory RNAs in Stem Cells: Methods and Protocols aims to accelerate progress in this crucial field by reducing the time required to decipher and put into practice procedures published in the literature.

Editors and Affiliations

  • Department of Biology, East Carolina University, Greenville, USA

    Baohong Zhang

Bibliographic Information

  • Book Title: RNAi and Small Regulatory RNAs in Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Baohong Zhang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7108-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2017

  • Hardcover ISBN: 978-1-4939-7106-0Published: 03 July 2017

  • Softcover ISBN: 978-1-4939-8386-5Published: 12 June 2018

  • eBook ISBN: 978-1-4939-7108-4Published: 03 July 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 311

  • Number of Illustrations: 18 b/w illustrations, 32 illustrations in colour

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

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