Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1615)
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Table of contents (38 protocols)
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Back Matter
About this book
This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but arealso of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope.Editors and Affiliations
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Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ - CNRS, Marseille, France
Laure Journet, Eric Cascales
Bibliographic Information
Book Title: Bacterial Protein Secretion Systems
Book Subtitle: Methods and Protocols
Editors: Laure Journet, Eric Cascales
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7033-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-7031-5Published: 01 July 2017
Softcover ISBN: 978-1-4939-8374-2Published: 08 August 2018
eBook ISBN: 978-1-4939-7033-9Published: 01 July 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 528
Number of Illustrations: 26 b/w illustrations, 70 illustrations in colour
Topics: Microbiology
Industry Sectors: Biotechnology, Chemical Manufacturing, Consumer Packaged Goods, Pharma