3D Cell Culture

1. Micropatterning

   1. 3D Neural Culture in Dual Hydrogel Systems

      • J. Lowry Curley, Michael J. Moore

      Pages 225-237

   2. 3D Cell Culture in Micropatterned Hydrogels Prepared by Photomask, Microneedle, or Soft Lithography Techniques

      • Seyedsina Moeinzadeh, Esmaiel Jabbari

      Pages 239-252

   3. 3D Stem Cell Niche Engineering via Two-Photon Laser Polymerization

      • Michele M. Nava, Tommaso Zandrini, Giulio Cerullo, Roberto Osellame, Manuela T. Raimondi

      Pages 253-266

2. Microfluidic Approaches for 3D Cell Culture

   1. Front Matter

      Pages 267-267

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   2. Microfluidic-Based Generation of 3D Collagen Spheres to Investigate Multicellular Spheroid Invasion

      • Fabien Bertillot, Youmna Attieh, Morgan Delarue, Basile G. Gurchenkov, Stephanie Descroix, Danijela Matic Vignjevic et al.

      Pages 269-279

   3. High-Throughput Cancer Cell Sphere Formation for 3D Cell Culture

      • Yu-Chih Chen, Euisik Yoon

      Pages 281-291

   4. High-Throughput 3D Tumor Culture in a Recyclable Microfluidic Platform

      • Wenming Liu, Jinyi Wang

      Pages 293-301

   5. High-Throughput Microfluidic Platform for 3D Cultures of Mesenchymal Stem Cells

      • Paola Occhetta, Roberta Visone, Marco Rasponi

      Pages 303-323

   6. 3D Anastomosed Microvascular Network Model with Living Capillary Networks and Endothelial Cell-Lined Microfluidic Channels

      • Xiaolin Wang, Duc T. T. Phan, Steven C. George, Christopher C. W. Hughes, Abraham P. Lee

      Pages 325-344

   7. Human Lung Small Airway-on-a-Chip Protocol

      • Kambez H. Benam, Marc Mazur, Youngjae Choe, Thomas C. Ferrante, Richard Novak, Donald E. Ingber

      Pages 345-365

3. Bioprinting

   1. Front Matter

      Pages 367-367

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   2. Microfluidic Bioprinting of Heterogeneous 3D Tissue Constructs

      • Cristina Colosi, Marco Costantini, Andrea Barbetta, Mariella Dentini

      Pages 369-380

   3. Bioprinting of 3D Tissue Models Using Decellularized Extracellular Matrix Bioink

      • Falguni Pati, Dong-Woo Cho

      Pages 381-390

   4. Bioprinting Cartilage Tissue from Mesenchymal Stem Cells and PEG Hydrogel

      • Guifang Gao, Karen Hubbell, Arndt F. Schilling, Guohao Dai, Xiaofeng Cui

      Pages 391-398

4. Imaging and Image Analysis of 3D Cell Cultures

   1. Front Matter

      Pages 399-399

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   2. Real-Time Cell Cycle Imaging in a 3D Cell Culture Model of Melanoma

      • Loredana Spoerri, Kimberley A. Beaumont, Andrea Anfosso, Nikolas K. Haass

      Pages 401-416

   3. Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy

      • Josef Jaros, Michal Petrov, Marketa Tesarova, Ales Hampl

      Pages 417-431

   4. Quantitative Phenotypic Image Analysis of Three-Dimensional Organotypic Cultures

      • Malin Åkerfelt, Mervi Toriseva, Matthias Nees

      Pages 433-445

5. Erratum to: Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional Hydrogels

   • Massimiliano Caiazzo, Yoji Tabata, Matthias P. Lutolf

   Pages E1-E1

6. Back Matter

   Pages 447-452

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This book provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic approaches and emerging 3D bioprinting techniques, which are used in developmental, stem cell, cancer, and pharmacological studies, among many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and cutting-edge, 3D Cell Culture: Methods and Protocols aims to inspire researchers to develop novel 3D cell culture techniques according to their specific scientific needs and interests, leading to a new generation of physiologically relevant and realistic 3D cell cultures.




Chapter 15 of this book is available open access under a CC BY 4.0 license.

• Hydrogels
• 3D Organoids
• Scaffolds
• Organotypic cultures
• Micropatterning
• Microfluidics
• Bioprinting
• Three-dimensional cell culture

• Department of Histology and Embryology, Masaryk University, Brno, Czech Republic

  Zuzana Koledova

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