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Telomeres and Telomerase

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1587)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
  2. Introduction to Telomeres and Telomerase

    • Zhou Songyang
    Pages 1-13
  3. Telomere Length Analysis by Quantitative Fluorescent in Situ Hybridization (Q-FISH)

    • Isabelle Ourliac-Garnier, Arturo Londoño-Vallejo
    Pages 29-39
  4. Telomere Strand-Specific Length Analysis by Fluorescent In Situ Hybridization (Q-CO-FISH)

    • Isabelle Ourliac-Garnier, Arturo Londoño-Vallejo
    Pages 41-54
  5. Telomere G-Rich Overhang Length Measurement: DSN Method

    • Yong Zhao, Jerry W. Shay, Woodring E. Wright
    Pages 55-62
  6. Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In

    • Yong Zhao, Jerry W. Shay, Woodring E. Wright
    Pages 71-82
  7. Telomeres and NextGen CO-FISH: Directional Genomic Hybridization (Telo-dGHâ„¢)

    • Miles J. McKenna, Erin Robinson, Edwin H. Goodwin, Michael N. Cornforth, Susan M. Bailey
    Pages 103-112
  8. Visualization of Human Telomerase Localization by Fluorescence Microscopy Techniques

    • Eladio Abreu, Rebecca M. Terns, Michael P. Terns
    Pages 113-125
  9. Cytogenetic Analysis of Telomere Dysfunction

    • Rekha Rai, Asha S. Multani, Sandy Chang
    Pages 127-131
  10. Probing the Telomere Damage Response

    • Rekha Rai, Sandy Chang
    Pages 133-138
  11. In Vitro Preparation and Crystallization of Vertebrate Telomerase Subunits

    • Jing Huang, Christopher J. Bley, Dustin P. Rand, Julian J. L. Chen, Ming Lei
    Pages 161-169
  12. Analysis of Telomere Proteins by Chromatin Immunoprecipitation (ChIP)

    • Feng Liu, Xuyang Feng, Wenbin Ma
    Pages 205-214

About this book

This third edition volume expands on the previous editions with new and improved assays that ask essential questions of telomere regulation in diverse model systems. The chapters in this book discuss genetic, proteomic, genomic, biochemical, and molecular approaches that provide insight into the complex protein interaction networks active in the telomere chromatin. This book also details information regarding telomere dynamics in response to stress or stimuli. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Telomeres and Telomerase: Methods and Protocols, Third Edition is a valuable and informative resource for anyone who is interested in the developing field of telomeres and telomerase.

Editors and Affiliations

  • Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, USA

    Zhou Songyang

Bibliographic Information

  • Book Title: Telomeres and Telomerase

  • Book Subtitle: Methods and Protocols

  • Editors: Zhou Songyang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6892-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6891-6Published: 21 March 2017

  • Softcover ISBN: 978-1-4939-8330-8Published: 09 June 2018

  • eBook ISBN: 978-1-4939-6892-3Published: 21 March 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 218

  • Number of Illustrations: 19 b/w illustrations, 18 illustrations in colour

  • Topics: Biochemistry, general

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access