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Techniques to Investigate Mitochondrial Function in Neurons

  • Stefan Strack
  • Yuriy M. Usachev

Part of the Neuromethods book series (NM, volume 123)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Ronald A. Merrill, Kyle H. Flippo, Stefan Strack
    Pages 31-48
  3. Meredith M. Course, Chung-Han Hsieh, Pei-I Tsai, Jennifer A. Codding-Bui, Atossa Shaltouki, Xinnan Wang
    Pages 49-66
  4. Liang Zhang, Eugenia Trushina
    Pages 95-113
  5. Lauren Y. Shields, Bryce A. Mendelsohn, Ken Nakamura
    Pages 115-131
  6. Jacob E. Rysted, Zhihong Lin, Yuriy M. Usachev
    Pages 151-178
  7. Karolina Can, Sebastian Kügler, Michael Müller
    Pages 179-197
  8. Tatiana Brustovetsky, Nickolay Brustovetsky
    Pages 239-248
  9. Gregory T. Macleod, Maxim V. Ivannikov
    Pages 279-297
  10. Kelly L. Stauch, Howard S. Fox
    Pages 299-319
  11. Lawrence R. Gray, Alix A. J. Rouault, Lalita Oonthonpan, Adam J. Rauckhorst, Julien A. Sebag, Eric B. Taylor
    Pages 321-338
  12. Back Matter
    Pages 339-344

About this book

Introduction

This volume describes a broad spectrum of experimental approaches for investigating structure, function, and transport of neuronal mitochondria in health and disease. Most of these approaches were only recently developed and range from electron tomography-based 3D reconstruction of mitochondrial cristae to patch clamp recording from mitochondria in intact neurons. The chapters in this book cover topics such as mitochondrial proteomics, fluorescence lifetime imaging, respirometry and mitophagy, as well as optical approaches based on the use of genetically engineered fluorescent sensors for monitoring synaptic ATP and axonal ROS generation, mitochondrial Ca2+ cycling and pH changes, and mitochondrial dynamics and axonal trafficking in live neurons. Each chapter also discusses difficulties, tips, tricks, and precautions to take. Neuromethods series style chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. 
Cutting-edge and comprehensive, Techniques to Investigate Mitochondrial Function in Neurons is a valuable and useful resource for a broad range of investigators interested in the function of neuronal mitochondria in health and disease states.

 

Keywords

function and transport of neuronal mitochondria disease electron tomography-based 3D reconstruction mitochondrial cristae patch clamp recording synaptic ATP axonal ROS generation axonal trafficking mass spectrometry

Editors and affiliations

  • Stefan Strack
    • 1
  • Yuriy M. Usachev
    • 2
  1. 1.Department of PharmacologyUniversity of Iowa Carver College of MedicineIowa CityUSA
  2. 2.Department of PharmacologyUniversity of Iowa Carver College of MedicineIowa CityUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6890-9
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6888-6
  • Online ISBN 978-1-4939-6890-9
  • Series Print ISSN 0893-2336
  • Series Online ISSN 1940-6045
  • Buy this book on publisher's site
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