Matrix Metalloproteases

Methods and Protocols

  • Charles A. Galea

Part of the Methods in Molecular Biology book series (MIMB, volume 1579)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Expression and Purification of Matrix Metalloproteases

    1. Front Matter
      Pages 1-1
    2. Krishna K. Singh, Ruchi Jain, Harini Ramanan, Deepak K. Saini
      Pages 3-16
  3. Structural Characterization of Matrix Metalloproteases

    1. Front Matter
      Pages 47-47
    2. Elena Decaneto, Wolfgang Lubitz, Hideaki Ogata
      Pages 49-60
    3. Tara C. Marcink, Rama K. Koppisetti, Yan G. Fulcher, Steven R. Van Doren
      Pages 61-86
    4. Louise E. Butt, Robert A. Holland, Nikul S. Khunti, Debra L. Quinn, Andrew R. Pickford
      Pages 87-108
  4. Computational Simulations of Matrix Metalloproteases

    1. Front Matter
      Pages 109-109
    2. Natalia Díaz, Dimas Suárez
      Pages 111-134
  5. Determining Matrix Metalloprotease Substrate Specificity

  6. Detection of Matrix Metalloproteases

    1. Front Matter
      Pages 229-229
    2. Rajeev B. Tajhya, Rutvik S. Patel, Christine Beeton
      Pages 231-244
    3. Harvey Hensley, Harry S. Cooper, Wen-Chi L. Chang, Margie L. Clapper
      Pages 245-255
  7. Matrix Metalloprotease Inhibitors

    1. Front Matter
      Pages 257-257

About this book

Introduction

Since the identification of the first matrix metalloprotease (insterstitial collagenase or MMP-1) more than 20 closely related and evolutionarily conserved vertebrate MMPs have been discovered. Chapters in this volume detail methods and protocols on recent advances in the expression and purification of MMPs, biophysical methods such as X-ray crystallography, NMR spectroscopy and small angle X-ray scattering, computational and experimental methods for determining MMP specificity, methods for detecting MMP activity in vitro and in vivo, and methods for the development and characterization of MMP-based inhibitors as potential therapeutics for the treatment of various diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Matrix Metalloproteases: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Keywords

matrix metalloprotease X-ray crystallography NMR spectroscopy small angle X-ray scattering enzymes substrate specificity in vitro in vivo

Editors and affiliations

  • Charles A. Galea
    • 1
  1. 1.Drug Delivery, Disposition and DynamicsMonash Institute of Pharmaceutical Sciences, Monash UniversityParkvilleAustralia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6863-3
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6861-9
  • Online ISBN 978-1-4939-6863-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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