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  • Book
  • © 2017

Thermogenic Fat

Methods and Protocols

Editors:

  1. Jun Wu

    1. Department of Molecular and Integrative Physiology, Life Sciences Institute, University of Michigan, Ann Arbor, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains keynotes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1566)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    PDF

  2. Isolation and Culture Methods

    1. Front Matter

      Pages 1-1
      PDF

    3. Isolation of Mouse Stromal Vascular Cells for Monolayer Culture

      • Longhua Liu, Louise D. Zheng, Sarah R. Donnelly, Margo P. Emont, Jun Wu, Zhiyong Cheng
      Pages 9-16

    4. Flow Cytometry Assisted Isolation of Adipose Tissue Derived Stem Cells

      • Umesh D. Wankhade, Sushil G. Rane
      Pages 17-24

    5. Flow Cytometric Isolation and Differentiation of Adipogenic Progenitor Cells into Brown and Brite/Beige Adipocytes

      • Jochen Steinbring, Antonia Graja, Anne-Marie Jank, Tim J. Schulz
      Pages 25-36

    6. Immuno-Magnetic Isolation and Thermogenic Differentiation of White Adipose Tissue Progenitor Cells

      • Rohollah Babaei, Irem Bayindir-Buchhalter, Irina Meln, Alexandros Vegiopoulos
      Pages 37-48

    8. Differentiation and Metabolic Interrogation of Human Adipocytes

      • Nicki A. Baker, Lindsey A. Muir, Carey N. Lumeng, Robert W. O’Rourke
      Pages 61-76

  3. Functional Analysis, Bioengineering, and Other Applications

    1. Front Matter

      Pages 87-87
      PDF

    3. Genetic Mouse Models: The Powerful Tools to Study Fat Tissues

      • Xingxing Kong, Kevin W. Williams, Tiemin Liu
      Pages 99-107

    5. Bioenergetic Analyses in Adipose Tissue

      • Lawrence Kazak
      Pages 125-134

    7. Isolation and Patch-Clamp of Primary Adipocytes

      • Yanhui Zhang, Dan Tong, Anil Mishra, Litao Xie, Isaac Samuel, Jessica K. Smith et al.
      Pages 145-150

    9. Identification and Quantification of Human Brown Adipose Tissue

      • Maria Chondronikola, Craig Porter, John O. Ogunbileje, Labros S. Sidossis
      Pages 159-176

    10. Designing 3-D Adipospheres for Quantitative Metabolic Study

      • Takeshi Akama, Brendan M. Leung, Joe Labuz, Shuichi Takayama, Tae-Hwa Chun
      Pages 177-183
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About this book

This volume presents a collection of protocols that describe methodologies to study thermogenic fat biology from various angles. This book is divided into 2 parts. Part 1 focuses on establishing in vitro culture systems. The chapters in this section introduce techniques on how to isolate, culture, and differentiate primary fat cells from both laboratory mice and humans. This part also presents flow cytometry methods to isolate various subpopulations of precursors within the stromal vascular fraction of the adipose tissue, which contains both preadipocytes and immune cells. Part 2 introduces multiple means to genetically manipulate and evaluate brown and beige fat in vivo. The chapters in this section explore methods on bioenergetics analyses both in vitro and in vivo. They also cover how to evaluate thermogenic fat contents and activity in humans, how to culture these cells though interdisciplinary approaches, and how to use thermogenic fat cell lines to carry out drug screens. Writtenin the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Thermogenic Fat: Methods and Protocols is a valuable resource for both experts and novices who are interested in learning assays and investigating brown and beige fat functions.

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Keywords

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Editors and Affiliations

  • Department of Molecular and Integrative Physiology, Life Sciences Institute, University of Michigan, Ann Arbor, USA

    Jun Wu

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Bibliographic Information

  • Book Title: Thermogenic Fat

  • Book Subtitle: Methods and Protocols

  • Editors: Jun Wu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6820-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2017

  • Hardcover ISBN: 978-1-4939-6819-0Published: 28 February 2017

  • Softcover ISBN: 978-1-4939-8308-7Published: 12 July 2018

  • eBook ISBN: 978-1-4939-6820-6Published: 26 February 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 214

  • Number of Illustrations: 12 b/w illustrations, 30 illustrations in colour

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

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Access via your institution

Buy it now

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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