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SH2 Domains

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Kazuya Machida

    1. Department of Genetics and Genome Sciences, University of Connecticut School of Medicine, Farmington, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Bernard A. Liu

    1. Broad Institute of MIT and Harvard, Cambridge, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1555)

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Table of contents (31 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Overview

    1. Front Matter

      Pages 1-1
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    2. Introduction: History of SH2 Domains and Their Applications

      • Bernard A. Liu, Kazuya Machida
      Pages 3-35

    3. What Have We Learned from SH2 Domains?

      • Bruce J. Mayer
      Pages 37-43

  4. Experimental Tools

    1. Front Matter

      Pages 115-115
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    2. Expression and Production of SH2 Domain Proteins

      • Bernard A. Liu, Mari Ogiue-Ikeda, Kazuya Machida
      Pages 117-162

    3. Expression and Purification of Soluble STAT5b/STAT3 Proteins for SH2 Domain Binding Assay

      • Akira Asai, Kazuyuki Takakuma
      Pages 163-172

    4. Purification of SOCS (Suppressor of Cytokine Signaling) SH2 Domains for Structural and Functional Studies

      • Nicholas P. D. Liau, Artem Laktyushin, Jeffrey J. Babon
      Pages 173-182

    5. Expression and Purification of SH2 Domains Using Baculovirus Expression System

      • Mari Ogiue-Ikeda, Kazuya Machida
      Pages 183-198

    6. Functionally Altered SH2 Domains for Biochemical Studies: Loss-of-Function Mutant and Domain Concatenation

      • Mari Ogiue-Ikeda, Kazuya Machida
      Pages 199-223

    7. Creation of Phosphotyrosine Superbinders by Directed Evolution of an SH2 Domain

      • Haiming Huang, Tomonori Kaneko, Sachdev S. Sidhu, Shawn S. C. Li
      Pages 225-254

  5. Structural Analysis and Binding Assays

    1. Front Matter

      Pages 255-255
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    2. Structural Characterization of Monomeric/Dimeric State of p59fyn SH2 Domain

      • Radu Huculeci, Fabien Kieken, Abel Garcia-Pino, Lieven Buts, Nico van Nuland, Tom Lenaerts
      Pages 257-267

    3. NMR Chemical Shift Mapping of SH2 Peptide Interactions

      • Marissa A. McKercher, Deborah S. Wuttke
      Pages 269-290
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Keywords

About this book

This volume covers an array of techniques available for studying SH2 domains and phosphotyrosine signaling. The book is divided into six parts: Part I outlines the history of SH2, technology development, and cell signaling; Part II focuses on computational approaches and tools used for identification, classification, and predictions of SH2 domain binding partners; Part III details various ways to prepare the SH2 domains as experimental reagents; Part IV presents methods for structural analysis and conventional binding assays using SH2 domains; Part V describes high-throughput and proteomics approaches to aid in analyzing SH2-mediated interactions; and Part VI covers applications for SH2 domain to functional and imaging analyzes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and thorough, SH2 Domains: Methods and Protocols is a valuable resource for computational biologists, biochemists, structural biologists, cell biologists, pathologists, and people interested in SH2 domains and phosphotyrosine signaling. Researchers who are investigating how protein interaction domain mediate specificity in signaling systems may also find this book informative.

Editors and Affiliations

  • Department of Genetics and Genome Sciences, University of Connecticut School of Medicine, Farmington, USA

    Kazuya Machida

  • Broad Institute of MIT and Harvard, Cambridge, USA

    Bernard A. Liu

About the editors

Kazuya Machida, MD, PhD Dept of Genetics and Developmental Biology Univ of Connecticut Health Center Cell and Genome Sciences, Farmington, CT, USA
Tel: 860-679-1830; Email: machida@neuron.uchc.edu

Bibliographic Information

  • Book Title: SH2 Domains

  • Book Subtitle: Methods and Protocols

  • Editors: Kazuya Machida, Bernard A. Liu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6762-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6760-5Published: 15 January 2017

  • Softcover ISBN: 978-1-4939-8295-0Published: 09 June 2018

  • eBook ISBN: 978-1-4939-6762-9Published: 14 January 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 558

  • Number of Illustrations: 39 b/w illustrations, 79 illustrations in colour

  • Topics: Protein Science

  • Industry Sectors: Biotechnology, Pharma

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