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Synapse Development

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Alexandros Poulopoulos

    1. Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, USA

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  • Includes cutting-edge methods and protocols for the study of synapse development
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1538)

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Table of contents (23 protocols)

  2. Revealing Patterns of Synaptic Connectivity

    1. Front Matter

      Pages 291-291
      Download chapter PDF

    2. Using Fluorescent Markers to Estimate Synaptic Connectivity In Situ

      • Mrinalini Hoon, Raunak Sinha, Haruhisa Okawa
      Pages 293-320

    3. Dual Anterograde and Retrograde Viral Tracing of Reciprocal Connectivity

      • Matthias G. Haberl, Melanie Ginger, Andreas Frick
      Pages 321-340

    4. Mapping Synaptic Inputs of Developing Neurons Using Calcium Imaging

      • Johan Winnubst, Christian Lohmann
      Pages 341-352

    5. Monosynaptic Tracing in Developing Circuits Using Modified Rabies Virus

      • Laura Cocas, Gloria Fernandez
      Pages 353-366

  3. Back Matter

    Pages 367-372
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Keywords

About this book

This detailed volume collects protocols for experimentation into how neurons connect to produce the extraordinary functionalities of the nervous system. Contributed by experts and pioneers in their respective techniques, the book covers synapses in the brain and in culture, their constituents, their structures, their dynamics, and the assemblies they form, all in the structure of a laboratory guide. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and  avoiding known pitfalls. 

Authoritative and practical, Synapse Development: Methods and Protocols serves as an ideal guide to minimizing the barrier to entry for the integration of new approaches with existing expertise, producing syntheses that will foster novel perspectives on the many ways in which synapses form, transform, and transmit.

Editors and Affiliations

  • Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, USA

    Alexandros Poulopoulos

Bibliographic Information

  • Book Title: Synapse Development

  • Book Subtitle: Methods and Protocols

  • Editors: Alexandros Poulopoulos

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6688-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6686-8Published: 10 December 2016

  • Softcover ISBN: 978-1-4939-8273-8Published: 06 July 2018

  • eBook ISBN: 978-1-4939-6688-2Published: 10 December 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 372

  • Number of Illustrations: 12 b/w illustrations, 67 illustrations in colour

  • Topics: Neurosciences

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Health & Hospitals, Pharma

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