Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1537)
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Table of contents (29 protocols)
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Front Matter
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Saliva and Other Oral Fluids
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Front Matter
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Molecular Biosciences
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Front Matter
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About this book
This second edition volume presents a selection of cellular and molecular techniques that can be adapted to cover a range of applications and diseases. The book is divided into three sections: saliva and oral diseases, molecular biosciences, and cell and tissues. The first section contains chapters that discuss proteomic analyses by mass spectrometry and NMR-based metabolomics that can be used to not only study saliva, but also to assess other oral fluids such as gingival crevicular fluid. The second section contains chapters that profile oral microbial communities, quantitative real-time PCR, and adhesion of yeast and bacteria to oral surfaces. The third section deals with a range of approaches that enable the behavior of cells and tissues in both health and disease to be analyzed at the molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Oral Biology: Molecular Techniques and Applications, Second Edition is a useful resource for oral biologists at all levels (students, early career researchers, and experienced veterans), and it provides a ready reference to new techniques and approaches that can be used to answer numerous specific scientific questions that may lead to a deeper understanding and treatments of oral diseases.
Editors and Affiliations
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University of Otago Fac. Dentistry, Dunedin, New Zealand
Gregory J. Seymour
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Department of Oral Sciences, University of Otago Faculty of Dentistry, Dunedin, New Zealand
Mary P. Cullinan
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Sir John Walsh Research Institute, University of Otago Faculty of Dentistry, Dunedin, New Zealand
Nicholas C.K. Heng
Bibliographic Information
Book Title: Oral Biology
Book Subtitle: Molecular Techniques and Applications
Editors: Gregory J. Seymour, Mary P. Cullinan, Nicholas C.K. Heng
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6685-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-6683-7Published: 07 December 2016
Softcover ISBN: 978-1-4939-8272-1Published: 07 July 2018
eBook ISBN: 978-1-4939-6685-1Published: 05 December 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XIV, 503
Number of Illustrations: 38 b/w illustrations, 54 illustrations in colour
Topics: Molecular Medicine
Industry Sectors: Biotechnology, Chemical Manufacturing, Consumer Packaged Goods, Health & Hospitals, Pharma