Epstein Barr Virus

Methods and Protocols

  • Janos Minarovits
  • Hans Helmut Niller

Part of the Methods in Molecular Biology book series (MIMB, volume 1532)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Janos Minarovits, Hans Helmut Niller
    Pages 1-32
  3. Hans-Helmut Niller, Georg Bauer
    Pages 33-55
  4. Rachel M. Temple, Craig Meyers, Clare E. Sample
    Pages 65-78
  5. Tina O’Grady, Melody Baddoo, Erik K. Flemington
    Pages 105-121
  6. Gulfaraz Khan, Pretty S. Philip
    Pages 159-167
  7. Ferenc Bánáti, Anita Koroknai, Kálmán Szenthe
    Pages 169-177
  8. Ferenc Bánáti, Kálmán Szenthe
    Pages 215-227
  9. Ken-Ichi Imadome, Shigeyoshi Fujiwara
    Pages 241-254
  10. Lauren P. McLaughlin, Stephen Gottschalk, Cliona M. Rooney, Catherine M. Bollard
    Pages 255-265

About this book

Introduction

This volume explores data from the applications of molecular biological methods and the applications of recent immunological and cytogenetic methods in Epstein-Barr Virus (EBV) that will offer readers possible new solutions to the unresolved problems in the EBV field. Chapters in this book cover topics such as: viral life cycle, latency, EBV-associated diseases and EBV diagnostics; in vitro methods including organotypic cultures for the analysis of EBV-epithelial cell interactions; identification of the interacting viral and cellular proteins using affinity purification-mass spectrometry methods; 3D telomere FISH; transcription analysis using high-throughput RNA sequencing, qPCR and nuclear run-on assay; analysis of viral and cellular microRNAs; isolation and characterization of exosomes and the assessment of their function; characterization of the viral genome by terminal repeat analysis and sequencing; the use of chromatin immunoprecipitation coupled sequencing (ChIP-Seq) for the analysis of Zta-DNA interactions; epigenetic analysis by bisulfite sequencing and ChIP; novel in vivo models for the study of EBV infection; and how immunological, virological, tissue culture and molecular methods can be combined to yield Good Manufacturing Practice-compliant EBV-specific T cells for the immunotherapy of EBV-associated post-transplant lymphoproliferative diseases (PTLD). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Epstein Barr-Virus: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.

Keywords

EBV-associated diseases EBV-epithelial cell interactions purification-mass spectrometry methods 3D telomere FISH high-throughput RNA sequencing qPCR and nuclear run-on assay chromatin immunoprecipitation coupled sequencing (ChIP-Seq) Zta-DNA interactions tissue culture post-transplant lymphoproliferative diseases (PTLD) Good Manufacturing Practice-complaint

Editors and affiliations

  • Janos Minarovits
    • 1
  • Hans Helmut Niller
    • 2
  1. 1.University of SzegedSzegedHungary
  2. 2.University of RegensburgRegensburgGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6655-4
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6653-0
  • Online ISBN 978-1-4939-6655-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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