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Phagocytosis and Phagosomes

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1519)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xii
  2. Phagocytosis: Hungry, Hungry Cells

    • Matthew Gray, Roberto J. Botelho
    Pages 1-16
  3. Analysis of Human and Mouse Neutrophil Phagocytosis by Flow Cytometry

    • Noah Fine, Oriyah Barzilay, Michael Glogauer
    Pages 17-24
  4. Quantitative Efferocytosis Assays

    • Amanda L. Evans, Jack W. D. Blackburn, Charles Yin, Bryan Heit
    Pages 25-41
  5. Quantifying Phagocytosis by Immunofluorescence and Microscopy

    • Christopher H. Choy, Roberto J. Botelho
    Pages 43-53
  6. Quantitative Live-Cell Fluorescence Microscopy During Phagocytosis

    • Stella M. Lu, Sergio Grinstein, Gregory D. Fairn
    Pages 79-91
  7. Intracellular Manipulation of Phagosomal Transport and Maturation Using Magnetic Tweezers

    • Shashank Shekhar, Vinod Subramaniam, Johannes S. Kanger
    Pages 93-112
  8. Quantitative Immunofluorescence to Study Phagosome Maturation

    • Roya M. Dayam, Roberto J. Botelho
    Pages 113-123
  9. Analysis of LC3-Associated Phagocytosis and Antigen Presentation

    • Laure-Anne Ligeon, Susana Romao, Christian Münz
    Pages 145-168
  10. Quantitative Spatiotemporal Analysis of Phagosome Maturation in Live Cells

    • Laura Schnettger, Maximiliano G. Gutierrez
    Pages 169-184
  11. Measuring Phagosomal pH by Fluorescence Microscopy

    • Johnathan Canton, Sergio Grinstein
    Pages 185-199
  12. Image-Based Analysis of Phagocytosis: Measuring Engulfment and Internalization

    • Nicholas D. Condon, Adam A. Wall, Jeremy C. Yeo, Nicholas A. Hamilton, Jennifer L. Stow
    Pages 201-214
  13. Isolation and Western Blotting of Latex-Bead Phagosomes to Track Phagosome Maturation

    • Anetta Härtlova, Julien Peltier, Orsolya Bilkei-Gorzo, Matthias Trost
    Pages 241-248
  14. Assessing the Phagosome Proteome by Quantitative Mass Spectrometry

    • Julien Peltier, Anetta Härtlova, Matthias Trost
    Pages 249-263
  15. Dissecting Phagocytic Removal of Apoptotic Cells in Caenorhabditis elegans

    • Shiya Cheng, Kai Liu, Chonglin Yang, Xiaochen Wang
    Pages 265-284
  16. Measurement of Salmonella enterica Internalization and Vacuole Lysis in Epithelial Cells

    • Jessica A. Klein, TuShun R. Powers, Leigh A. Knodler
    Pages 285-296

About this book

This volume details experimental approaches used to investigate phagocytosis and phagosome maturation. Chapters present methods and protocols on quantifying uptake and phagosome maturation using biophysical and biochemical approaches, proteomics, microscopy, and flow cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Phagocytosis and Phagosomes: Methods and Protocols aims to be an important resource for both experts in the field and for those investigators delving into phagocytosis and phagosome maturation for the first time.

Editors and Affiliations

  • Department of Chemistry and Biology, Ryerson University, Toronto, Canada

    Roberto Botelho

Bibliographic Information

  • Book Title: Phagocytosis and Phagosomes

  • Book Subtitle: Methods and Protocols

  • Editors: Roberto Botelho

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6581-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6579-3Published: 04 November 2016

  • Softcover ISBN: 978-1-4939-8241-7Published: 08 June 2018

  • eBook ISBN: 978-1-4939-6581-6Published: 04 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 360

  • Number of Illustrations: 33 b/w illustrations, 24 illustrations in colour

  • Topics: Microbiology

  • Industry Sectors: Biotechnology, Chemical Manufacturing, Consumer Packaged Goods, Pharma

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access