Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1503)
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Table of contents (19 chapters)
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Back Matter
Keywords
About this book
This volume discusses detailed protocols for the analysis of glycosylation at the level of free glycans and glycopeptides. The book covers topics such as the importance of glycans in eukaryotic life (with a focus on mammals and particularly humans); recent technologies that allow the characterization of larger sets of samples; and method repeatability and robustness, as well as higher throughout with respect to sample preparation, measurement and data analysis. The chapters spans a wide range of techniques, including the analysis of fluorescently labeled glycans with HPLC, LC-MS analysis of glycopeptides both for glycosylation profiling and for in-depth tandem mass spectrometric analysis of protein glycosylation, and the analysis of glycans with fluorescent labeling, capillary electrophoresis in conjunction with laser induced fluorescence detection. The chapters also detail specific samples types including brain tissues, N-glycans from in-vitro cell cultures, milk oligosaccharides, invertebrate and protest N-glycans, as well as plant glycans. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and thorough, High-Throughout Glycomics and Glycoproteomics: Methods and Protocols is an essential reference for researchers planning to enter this rapidly evolving field.
Editors and Affiliations
Bibliographic Information
Book Title: High-Throughput Glycomics and Glycoproteomics
Book Subtitle: Methods and Protocols
Editors: Gordan Lauc, Manfred Wuhrer
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6493-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2017
Hardcover ISBN: 978-1-4939-6491-8Published: 14 October 2016
Softcover ISBN: 978-1-4939-8217-2Published: 27 June 2018
eBook ISBN: 978-1-4939-6493-2Published: 14 October 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 274
Number of Illustrations: 9 b/w illustrations, 42 illustrations in colour
Topics: Protein Science
Industry Sectors: Biotechnology, Pharma