Plant Synthetic Promoters

Methods and Protocols

  • Reinhard Hehl

Part of the Methods in Molecular Biology book series (MIMB, volume 1482)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Stefan M. Fischer, Alexander Böser, Jan P. Hirsch, Dierk Wanke
    Pages 49-66
  3. Ralf Stracke, Katharina Thiedig, Melanie Kuhlmann, Bernd Weisshaar
    Pages 67-81
  4. Elisabeth Wischnitzki, Kornel Burg, Maria Berenyi, Eva Maria Sehr
    Pages 83-102
  5. Tou Cheu Xiong, Frédéric Sanchez, Jean-François Briat, Frédéric Gaymard, Christian Dubos
    Pages 103-110
  6. Dipak Kumar Sahoo, Shayan Sarkar, Indu B. Maiti, Nrisingha Dey
    Pages 111-138
  7. Zsolt Kelemen, Jonathan Przybyla-Toscano, Nicolas Tissot, Loïc Lepiniec, Christian Dubos
    Pages 139-149
  8. Johanne Thévenin, Wenjia Xu, Louise Vaisman, Loïc Lepiniec, Bertrand Dubreucq, Christian Dubos
    Pages 151-161
  9. Konstantin Kanofsky, Mona Lehmeyer, Jutta Schulze, Reinhard Hehl
    Pages 163-174
  10. Elisa Fiume, Niek de Klein, Seung Yon Rhee, Enrico Magnani
    Pages 175-188
  11. Anna Coll, Mandy L. Wilson, Kristina Gruden, Jean Peccoud
    Pages 219-232
  12. Julio Bolívar, Reinhard Hehl, Lorenz Bülow
    Pages 247-257
  13. Bruno Contreras-Moreira, Jaime A. Castro-Mondragon, Claire Rioualen, Carlos P. Cantalapiedra, Jacques van Helden
    Pages 279-295
  14. Jaime A. Castro-Mondragon, Claire Rioualen, Bruno Contreras-Moreira, Jacques van Helden
    Pages 297-322

About this book

Introduction

This book assembles experimental and bioinformatics protocols for the design and experimental testing of synthetic promoters. The identification of cis-regulatory elements potentially achieving the desired expression of a gene is at the core of synthetic promoter design. For this, several bioinformatics chapters are presented. The experimental verification of the proposed expression profile conferred by the cis-regulatory elements requires the assembly of synthetic promoters. Several chapters are dedicated to the assembly of synthetic promoters, also including specific software tools to facilitate promoter design. Transient and transgenic reporter gene technology is a prominent approach to test the spatial and temporal expression driven by synthetic promoters, and several chapters address this approach. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice to ensure successful results in the lab. 

Practical and cutting-edge, Plant Synthetic Promoters: Methods and Protocols covers all steps required from the identification of cis-regulatory elements, over synthetic promoter design, to the experimental analysis of synthetic promoter function.

Keywords

Gene expression regulation Bioinformatics cis-regulatory elements Reporter genes Transcription factors Synthetic promoter function

Editors and affiliations

  • Reinhard Hehl
    • 1
  1. 1.Institut für GenetikTechnische Universität BraunschweigBraunschweigGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6396-6
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6394-2
  • Online ISBN 978-1-4939-6396-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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