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  • © 2016

Bioluminescence

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1461)

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Table of contents (25 protocols)

  1. Fabrication of Bioluminescent Probes

  2. Applications to Living Subjects and Instrumentations

    1. Front Matter

      Pages 225-225
    2. In Vivo Bioluminescent Imaging of ATP-Binding Cassette Transporter-Mediated Efflux at the Blood–Brain Barrier

      • Joshua Bakhsheshian, Bih-Rong Wei, Matthew D. Hall, R. Mark Simpson, Michael M. Gottesman
      Pages 227-239
    3. Theranostic Imaging of Cancer Gene Therapy

      • Thillai V. Sekar, Ramasamy Paulmurugan
      Pages 241-254
    4. Development of a Multicolor Bioluminescence Imaging Platform to Simultaneously Investigate Transcription Factor NF-κB Signaling and Apoptosis

      • Vicky T. Knol-Blankevoort, Laura Mezzanotte, Martijn J. W. E. Rabelink, Clemens W. G. M. Löwik, Eric L. Kaijzel
      Pages 255-270
    5. A Multichannel Bioluminescence Determination Platform for Bioassays

      • Sung-Bae Kim, Ryuichi Naganawa
      Pages 271-278
    6. A Bioluminescence Assay System for Imaging Metal Cationic Activities in Urban Aerosols

      • Sung-Bae Kim, Ryuichi Naganawa, Shingo Murata, Takayoshi Nakayama, Simon Miller, Toshiya Senda
      Pages 279-287
  3. Back Matter

    Pages 311-314

About this book

This volume presents detailed laboratory protocols regarding the three major route technologies luciferases, efficient optical probes, and applications to visualizing molecular events in living subjects. Bioluminescence: Methods and Protocols, Third Edition guides readers through chapters on ingredients of bioluminescent probes, and fabrication of bioluminescent probes, applications to living subjects and instrumentations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Bioluminescence: Methods and Protocols, Third Edition is a useful complement to the first and second edition for new and experienced researchers alike.

 

Editors and Affiliations

  • Natl Inst of AIST Res Inst for Envt Mgt Tech, Tsukuba, Japan

    Sung Bae Kim

Bibliographic Information

  • Book Title: Bioluminescence

  • Book Subtitle: Methods and Protocols

  • Editors: Sung Bae Kim

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3813-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3811-7Published: 18 July 2016

  • Softcover ISBN: 978-1-4939-8146-5Published: 31 May 2018

  • eBook ISBN: 978-1-4939-3813-1Published: 17 July 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XIII, 314

  • Number of Illustrations: 16 b/w illustrations, 84 illustrations in colour

  • Topics: Biochemistry, general

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access