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Cilia

Methods and Protocols

  • Peter Satir
  • Søren Tvorup Christensen

Part of the Methods in Molecular Biology book series (MIMB, volume 1454)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Daisuke Takao, Kristen J. Verhey
    Pages 1-14
  3. Patricia Verdier, Stine K. Morthorst, Lotte B. Pedersen
    Pages 15-33
  4. Rania Ghossoub, Louise Lindbæk, Anahi Molla-Herman, Alain Schmitt, Søren Tvorup Christensen, Alexandre Benmerah
    Pages 35-51
  5. Birgit Hegner Satir, Olatz Pampliega
    Pages 53-67
  6. Megan Smith Valentine, Judith L. Van Houten
    Pages 149-168
  7. T. Tony Yang, Weng Man Chong, Jung-Chi Liao
    Pages 169-192
  8. Frank P. Macaluso, Geoffrey S. Perumal, Johan Kolstrup, Peter Satir
    Pages 193-202
  9. Tamara Caspary, Daniela Marazziti, Nicolas F. Berbari
    Pages 203-214
  10. Swadhin Chandra Jana, Susana Mendonça, Sascha Werner, Monica Bettencourt-Dias
    Pages 215-236
  11. Emily L. Hunter, Winfield S. Sale, Lea M. Alford
    Pages 237-243
  12. Stephen M. King, Ramila S. Patel-King
    Pages 245-254
  13. Back Matter
    Pages 255-259

About this book

Introduction

This volume is the result of an explosion of molecular-based research on Cilia, which began with the discovery of the universality of intraflagellar transport (IFT) and ciliary genomics/proteomics. The chapters in this book cover topics such as: high resolution imaging and functional characterization of sensory and primary cilia in mammalian cells and zebrafish, methods to study ciliary-mediated chemoresponse in Paramecium, and methods to study centrosomes and cilia in C. elegans and Drosophila. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and cutting-edge, Cilia: Methods and Protocols is broad and covers motile, sensory, and primary cells. It is a valuable resource to anyone interested in entering the field of ciliary biology using model organisms, including flagellate algae, ciliates, planaria, nematodes, insects, zebrafish, and mammalian cells.

 

Keywords

intraflagellar transport (IFT) cell organelle motile cilia sensory cilia flagellate algae ciliates planaria nematodes Drosophila zebrafish Xenopus

Editors and affiliations

  • Peter Satir
    • 1
  • Søren Tvorup Christensen
    • 2
  1. 1.Albert Einstein College of MedicineYeshiva UniversityBronxUSA
  2. 2.Department of BiologyUniversity of CopenhagenCopenhagenDenmark

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-3789-9
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3787-5
  • Online ISBN 978-1-4939-3789-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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