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High Throughput Screening

Methods and Protocols

  • William P. Janzen

Part of the Methods in Molecular Biology book series (MIMB, volume 1439)

Table of contents

  1. Front Matter
    Pages i-xi
  2. David J. Powell, Robert P. Hertzberg, Ricardo Macarrόn
    Pages 1-32
  3. Mattias Rohman, Jonathan Wingfield
    Pages 47-63
  4. Chatura Jayakody, Emily A. Hull-Ryde
    Pages 99-113
  5. Xinyi Huang, Ann Aulabaugh
    Pages 115-130
  6. Chen-Ting Ma, Eduard A. Sergienko
    Pages 131-142
  7. Bianca Heedmann, Martin Klumpp
    Pages 159-169
  8. E. Lucile White, Nichole A. Tower, Lynn Rasmussen
    Pages 181-195
  9. Alberto di Silvio, JeanFrancois Rolland, Michela Stucchi
    Pages 197-206
  10. Wensheng Xie, Robert Silvers, Michael Ouellette, Zining Wu, Quinn Lu, Hu Li et al.
    Pages 207-226
  11. Dominique Perez, Peter C. Simons, Yelena Smagley, Larry A. Sklar, Alexandre Chigaev
    Pages 227-244
  12. David Murray, Lisa McWilliams, Mark Wigglesworth
    Pages 245-262
  13. Adam I. Fogel, Scott E. Martin, Samuel A. Hasson
    Pages 273-304
  14. Back Matter
    Pages 305-308

About this book

Introduction

This third edition volume expands on the previous editions both by presenting more detailed protocols for the techniques described in the first and second editions of High Throughput Screening: Methods and Protocols and by covering important new procedures. The first chapter of this book provides an overview of important assay development techniques, while the rest of the chapters detail how to develop and execute screens at whatever throughput the user needs. Some chapter examples are: structure-based virtual screening,  high throughput screening using mass spectrometry, identification of state-dependent blockers for voltage gated calcium channels, bioluminescence resonance energy transfer platform to monitor protein-protein interactions in live cells, high throughput flow cytometry, and application of imaging-based assays in microplate formats for high content screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, High Throughput Screening: Methods and Protocols, Third Edition, is a valuable resource for anyone who is interested in HTS research.

Keywords

high throughput screening assays complex cellular systems mass spectrometer techniques drug discovery compound libraries enzyme protein-protein interactions fluorescence polarization cell toxicity assays bioluminescence resonance energy transfer

Editors and affiliations

  • William P. Janzen
    • 1
  1. 1.Epizyme, Inc.CambridgeUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-3673-1
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3671-7
  • Online ISBN 978-1-4939-3673-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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