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Histone Deacetylases

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Sibaji Sarkar

    1. Boston University School of Medicine, Boston, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1436)

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Table of contents (22 protocols)

  1. Class III Deacetylases

    1. Molecular, Cellular, and Physiological Characterization of Sirtuin 7 (SIRT7)

      • Jiyung Shin, Danica Chen
      Pages 271-277

  2. Histone Deacetylase Inhibitors

    1. Front Matter

      Pages 279-279
      Download chapter PDF

    2. HDAC Inhibitors

      • Heidi Olzscha, Mina E. Bekheet, Semira Sheikh, Nicholas B. La Thangue
      Pages 281-303

    3. Assessment of the Antiproliferative Activity of a BET Bromodomain Inhibitor as Single Agent and in Combination in Non-Hodgkin Lymphoma Cell Lines

      • Elena Bernasconi, Eugenio Gaudio, Ivo Kwee, Francesco Bertoni
      Pages 305-312

    4. Screening the Impact of Sirtuin Inhibitors on Inflammatory and Innate Immune Responses of Macrophages and in a Mouse Model of Endotoxic Shock

      • Eleonora Ciarlo, Thierry Roger
      Pages 313-334

  3. Erratum to: Functional Analysis of Histone Deacetylase 11 (HDAC11)

    • Jie Chen, Eva Sahakian, John Powers, Maritza Lienlaf, Patricio Perez-Villarroel, Tessa Knox et al.
    Pages E1-E1

  4. Back Matter

    Pages 335-337
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Keywords

About this book

This volume provides different methodologies for all classes of histone deacetylases, which includes detailed procedures on Class I and II histone deacetylase inhibitors, SIRT inhibitors, and bromodomain inhibitors. Histone Deacetylases: Methods and Protocols is divided into four sections: Sections A and B describe methodologies used to detect the activity, function, or chromatin location of HDACs 1 through 11, with Section A discussing Class I and Section B discussing class II histone deacetylases; Section C focuses on the methodologies for cloning and characterizing the acetylation of SIRTs 1 through 7; and Section D describes methods related to histone deacetylase inhibitors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Histone Deacetylases: Methods and Protocols, is a valuable resource for investigators working on epigenetics, molecular biology, and genetics.

Reviews

“This volume of Methods in Molecular Biology, Histone deacetylases, provides comprehensive overview of the methods and procedures for analysis of histone modifications, namely histone deacetylation. … Methodological tools required for more detailed determination of the molecular and biological roles of distinct HDACs and HDAC-containing complexes are summarized in this book, which will no doubt hasten these important mechanistic studies.” (Bozena Smolkova, Neoplasma, Vol. 64 (3), 2017)

Editors and Affiliations

  • Boston University School of Medicine, Boston, USA

    Sibaji Sarkar

Bibliographic Information

  • Book Title: Histone Deacetylases

  • Book Subtitle: Methods and Protocols

  • Editors: Sibaji Sarkar

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3667-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3665-6Published: 01 June 2016

  • Softcover ISBN: 978-1-4939-8111-3Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3667-0Published: 31 May 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 337

  • Number of Illustrations: 21 b/w illustrations, 31 illustrations in colour

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

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