RNA-Protein Complexes and Interactions

Front Matter

Characterization of RNA–Protein Interactions: Lessons from Two RNA-Binding Proteins, SRSF1 and SRSF2

• Lindsey Skrdlant, Ren-Jang Lin

Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA Affinity Purification)

• Je-Hyun Yoon, Myriam Gorospe

Biotin–Streptavidin Affinity Purification of RNA–Protein Complexes Assembled In Vitro

• Shuai Hou, Lei Shi, Haixin Lei

Detecting RNA–Protein Interaction Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting

• Xuexiu Zheng, Sunghee Cho, Heegyum Moon, Tiing Jen Loh, Ha Na Jang, Haihong Shen

Purification of RNA–Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing Reaction Mixture

• Naoyuki Kataoka

Loading of Argonaute Protein with Small Duplex RNA in Cellular Extracts

• Keith T. Gagnon

In Vitro Analysis of Ribonucleoprotein Complex Remodeling and Disassembly

• Hua-Lin Zhou, Hua Lou

Single-Turnover Kinetics of Methyl Transfer to tRNA by Methyltransferases

• Ya-Ming Hou

Purification and Functional Reconstitution of Box H/ACA Ribonucleoprotein Particles

• Chao Huang, Guowei Wu, Yi-Tao Yu

Northwestern Blot Analysis: Detecting RNA–Protein Interaction After Gel Separation of Protein Mixture

• Shangbing Zang, Ren-Jang Lin

Measuring mRNA Translation by Polysome Profiling

• Marek Kudla, Fedor V. Karginov

Genome-Wide Profiling of RNA–Protein Interactions Using CLIP-Seq

• Cheryl Stork, Sika Zheng

Identification of Endogenous mRNA-Binding Proteins in Yeast Using Crosslinking and PolyA Enrichment

• Sarah F. Mitchell, Roy Parker

Ribo-Proteomics Approach to Profile RNA–Protein and Protein–Protein Interaction Networks

• Hsin-Sung Yeh, Jae-Woong Chang, Jeongsik Yong

Detection of Protein–Protein Interaction Within an RNA–Protein Complex Via Unnatural-Amino-Acid-Mediated Photochemical Crosslinking

• Fu-Lung Yeh, Luh Tung, Tien-Hsien Chang

Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX

• Jiehua Zhou, John J. Rossi

mCarts: Genome-Wide Prediction of Clustered Sequence Motifs as Binding Sites for RNA-Binding Proteins

• Sebastien M. Weyn-Vanhentenryck, Chaolin Zhang

Design of RNA-Binding Proteins: Manipulate Alternative Splicing in Human Cells with Artificial Splicing Factors

• Yang Wang, Zefeng Wang

Informational Suppression to Probe RNA:RNA Interactions in the Context of Ribonucleoproteins: U1 and 5′ Splice-Site Base-Pairing

• Jiazi Tan, Xavier Roca