Nonribosomal Peptide and Polyketide Biosynthesis

Methods and Protocols

  • Bradley S. Evans

Part of the Methods in Molecular Biology book series (MIMB, volume 1401)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Background and Overview

    1. Front Matter
      Pages 1-1
    2. Bradley R. Miller, Andrew M. Gulick
      Pages 3-29
    3. Marisa Till, Paul R. Race
      Pages 31-49
  3. In Vitro Methods

    1. Front Matter
      Pages 51-51
    2. Chitose Maruyama, Haruka Niikura, Masahiro Takakuwa, Hajime Katano, Yoshimitsu Hamano
      Pages 77-84
    3. Haoran Zhang, Lei Fang, Marcia S. Osburne, Blaine A. Pfeifer
      Pages 121-134
    4. Alexandra A. Soukup, Nancy P. Keller, Philipp Wiemann
      Pages 149-160
    5. Maria I. Vizcaino, Jason M. Crawford
      Pages 175-195
  4. Bioinformatic Methods

    1. Front Matter
      Pages 197-197
    2. Valérie Leclère, Tilmann Weber, Philippe Jacques, Maude Pupin
      Pages 209-232
    3. Nicolas Tremblay, Patrick Hill, Kyle R. Conway, Christopher N. Boddy
      Pages 233-252
    4. Guillermin Agüero-Chapin, Gisselle Pérez-Machado, Aminael Sánchez-Rodríguez, Miguel Machado Santos, Agostinho Antunes
      Pages 253-272

About this book

Introduction

This volume presents an overview of nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) structure and function.  It then continues with methods for the analysis of these pathways including conventional enzymological assays, contemporary mass spectrometric analysis techniques, specialized molecular biological approaches applicable to NRPSs and PKSs, and small molecule analysis tools tailored to this very special class of natural products, and concludes by examining bioinformatics tools for the analysis of these enzymes, pathways, and molecules. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Nonribosomal Peptide and Polyketide Biosynthesis: Methods and Protocols serves as a valuable reference for those experienced in studying NRPS and PKS enzymes, pathways, and natural products as well as a gateway for those just entering the field.

Keywords

Nonribosomal peptide synthetases (NRPSs) Polyketide synthases (PKSs) Biosynthetic enzymes Small molecule analysis Bioinformatics Enzymological assays Mass spectrometry

Editors and affiliations

  • Bradley S. Evans
    • 1
  1. 1.Donald Danforth Plant Science CenterSaint LouisUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-3375-4
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3373-0
  • Online ISBN 978-1-4939-3375-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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