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  • © 2016

Kidney Research

Experimental Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1397)

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Table of contents (19 protocols)

  1. Analytical Techniques

    1. Front Matter

      Pages 249-249
    2. Laser Capture Microdissection of Archival Kidney Tissue for qRT-PCR

      • Tim D. Hewitson, Michael Christie, Edward R. Smith
      Pages 251-265
    3. Using Phos-Tag in Western Blotting Analysis to Evaluate Protein Phosphorylation

      • Takahiro Horinouchi, Koji Terada, Tsunehito Higashi, Soichi Miwa
      Pages 267-277
    4. Qualitative and Quantitative Analysis of Histone Deacetylases in Kidney Tissue Sections

      • Katherine Ververis, Selly Marzully, Chrishan S. Samuel, Tim D. Hewitson, Tom C. Karagiannis
      Pages 279-289
  2. Back Matter

    Pages 291-292

About this book

This second edition expands upon the previous volume with additional emphasis on recent innovation in basic renal research and has a more holistic approach on associated disorders such as complications associated with peritoneal dialysis, ischemic acute kidney injury, the sympathetic nervous system, and vascular calcification. The book is divided into five parts: Part I provides a number of in vitro, in vivo, and ex vivo models of kidney disease and associated complications; Part II looks at recent advances in imaging techniques; Part III covers recent developments in studying metabolism in renal ischemia and reperfusion; Part IV addresses study and measurement of vascular calcification; and Part V explores analytical techniques that are both topical and of widespread relevance to the study of experimental renal disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Kidney Research: Experimental Protocols, Second Edition is a valuable collection of protocols useful to new and experienced researchers who are interested in the field of Nephrology.

Editors and Affiliations

  • Royal Hospital Melbourne Dept. Nephrology, Melbourne, Australia

    Tim D. Hewitson

  • Department of Nephrology, Royal Melbourne Hospital, Parkville, Melbourne, Australia

    Edward R. Smith

  • Department of Nephrology, Royal Melbourne Hospital, Parkville, Australia

    Stephen G. Holt

Bibliographic Information

  • Book Title: Kidney Research

  • Book Subtitle: Experimental Protocols

  • Editors: Tim D. Hewitson, Edward R. Smith, Stephen G. Holt

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3353-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3351-8Published: 17 December 2015

  • Softcover ISBN: 978-1-4939-8028-4Published: 29 March 2019

  • eBook ISBN: 978-1-4939-3353-2Published: 16 December 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 292

  • Number of Illustrations: 52 b/w illustrations, 14 illustrations in colour

  • Topics: Molecular Medicine, Nephrology, Pathology

  • Industry Sectors: Biotechnology, Chemical Manufacturing, Consumer Packaged Goods, Health & Hospitals, Pharma

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access