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  • Book
  • © 2016

Cancer Drug Resistance

Overviews and Methods

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1395)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiii
  2. Cancer Drug Resistance: A Brief Overview from a Genetic Viewpoint

    • José Rueff, António Sebastião Rodrigues
    Pages 1-18
  3. In Vitro Methods for Studying the Mechanisms of Resistance to DNA-Damaging Therapeutic Drugs

    • Pasarat Khongkow, Anna W. Middleton, Jocelyn P.-M. Wong, Navrohit K. Kandola, Mesayamas Kongsema, Gabriela Nestal de Moraes et al.
    Pages 39-53
  4. In Vitro Approaches to Study Regulation of Hepatic Cytochrome P450 (CYP) 3A Expression by Paclitaxel and Rifampicin

    • Romi Ghose, Pankajini Mallick, Guncha Taneja, Chun Chu, Bhagavatula Moorthy
    Pages 55-68
  5. Uptake and Permeability Studies to Delineate the Role of Efflux Transporters

    • Ramya Krishna Vadlapatla, Dhananjay Pal, Ashim K. Mitra
    Pages 69-74
  6. Dynamics of Expression of Drug Transporters: Methods for Appraisal

    • Marta Gromicho, José Rueff, António Sebastião Rodrigues
    Pages 75-85
  7. Fluorimetric Methods for Analysis of Permeability, Drug Transport Kinetics, and Inhibition of the ABCB1 Membrane Transporter

    • Ana Armada, Célia Martins, Gabriella Spengler, Joseph Molnar, Leonard Amaral, António Sebastião Rodrigues et al.
    Pages 87-103
  8. MicroRNAs and Cancer Drug Resistance

    • Bruno Costa Gomes, José Rueff, António Sebastião Rodrigues
    Pages 137-162
  9. The Role of MicroRNAs in Resistance to Current Pancreatic Cancer Treatment: Translational Studies and Basic Protocols for Extraction and PCR Analysis

    • Ingrid Garajová, Tessa Y. S. Le Large, Elisa Giovannetti, Geert Kazemier, Guido Biasco, Godefridus J. Peters
    Pages 163-187
  10. Methods for Studying MicroRNA Expression and Their Targets in Formalin-Fixed, Paraffin-Embedded (FFPE) Breast Cancer Tissues

    • Bruno Costa Gomes, Bruno Santos, José Rueff, António Sebastião Rodrigues
    Pages 189-205
  11. The Regulatory Role of Long Noncoding RNAs in Cancer Drug Resistance

    • Marjan E. Askarian-Amiri, Euphemia Leung, Graeme Finlay, Bruce C. Baguley
    Pages 207-227
  12. Cancer Exosomes as Mediators of Drug Resistance

    • Maria do Rosário André, Ana Pedro, David Lyden
    Pages 229-239
  13. Proteomics in the Assessment of the Therapeutic Response of Antineoplastic Drugs: Strategies and Practical Applications

    • Vukosava Milic Torres, Lazar Popovic, Fátima Vaz, Deborah Penque
    Pages 281-298
  14. Managing Drug Resistance in Cancer: Role of Cancer Informatics

    • Ankur Gautam, Kumardeep Chaudhary, Rahul Kumar, Sudheer Gupta, Harinder Singh, Gajendra P. S. Raghava
    Pages 299-312
  15. Erratum to: In Vitro Methods for Studying the Mechanisms of Resistance to DNA-Damaging Therapeutic Drugs

    • Pasarat Khongkow, Anna W. Middleton, Jocelyn P.-M. Wong, Navrohit K. Kandola, Mesayamas Kongsema, Gabriela Nestal de Moraes et al.
    Pages E1-E1
  16. Back Matter

    Pages 313-314

About this book

This volume discusses the latest advancements and technologies used in cancer drug resistance research. Cancer Drug Resistance: Overviews and Methods contains chapters that cover topics such as: studying the mechanics of resistance to DNA damaging therapeutic drugs; studies to delineate the role of efflux transporters; expression of drug transporters; resistance to targeted therapies in breast cancer; the role of microRNAs in current pancreatic cancer treatment; and cancer exosomes as mediators of drug resistance or clinical and molecular methods in drug development and the use of bioinformatics in the management of cancer drug resistance data. Written in the highly successful Methods in Molecular Biology series format, chapters include overviews of the main issues in cancer drug resistance and the respective mechanisms, as well as introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cancer Drug Resistance: Overviews and Methods, is a valuable resource to researchers, oncobiologists and clinical oncologists or anyone else who is interested in the study of cancer and its drug resistances.

Keywords

  • cellular resistance
  • multi-drug resistance (MDR)
  • DNA
  • chemotherapy
  • adenosine5'-triphosphate (ATP)
  • binding cassette (ABC) transporters
  • microRNAs
  • proteomics

Editors and Affiliations

  • New University of Lisbon, Lisbon, Portugal

    Jose Rueff, António Sebastião Rodrigues

Bibliographic Information

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access