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Advances and Clinical Practice in Pyrosequencing

  • Guohua Zhou
  • Qinxin Song

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Technology Preparation

    1. Front Matter
      Pages 1-1
    2. Huiyong Yang, Chao Liang, Zhiyao Chen, Bingjie Zou, Qinxin Song, Guohua Zhou
      Pages 3-12
    3. Yunlong Liu, Haiping Wu, Hui Ye, Zhiyao Chen, Qinxin Song, Bingjie Zou et al.
      Pages 13-21
    4. Qinxin Song, Huiyong Yang, Bingjie Zou, Hideki Kambara, Guohua Zhou
      Pages 23-30
    5. Qinxin Song, Haiping Wu, Guohua Zhou, Hideki Kambara
      Pages 31-39
    6. Huning Jia, Zhiyao Chen, Haiping Wu, Hui Ye, Bingjie Zou, Qinxin Song et al.
      Pages 41-49
    7. Pengfeng Xiao, Huan Huang, Bingjie Zou, Qinxin Song, Guohua Zhou, Zuhong Lu
      Pages 51-65
    8. Zhiyao Chen, Yunlong Liu, Hui Ye, Haiping Wu, Jinheng Li, Bingjie Zou et al.
      Pages 67-76
  3. Template Innovation

    1. Front Matter
      Pages 77-77
    2. Guohua Zhou, Tomoharu Kajiyama, Mari Gotou, Akihiko Kishimoto, Shigeya Suzuki, Hideki Kambara
      Pages 79-94
    3. Guohua Zhou, Masao Kamahori, Kazunori Okano, Gao Chuan, Kunio Harada, Hideki Kambara
      Pages 95-114
    4. Guohua Zhou, Hiromi Shirakura, Masao Kamahori, Kazunori Okano, Keiichi Nagai, Hideki Kambara
      Pages 115-128
    5. Guohua Zhou, Mari Gotou, Tomoharu Kajiyama, Hideki Kambara
      Pages 129-143
    6. Haiping Wu, Wenjuan Wu, Zhiyao Chen, Weipeng Wang, Bingjie Zou, Qinxin Song et al.
      Pages 145-154
    7. Qinxin Song, Xiemin Qi, Yiyun Shen, Yiru Jin, Bingjie Zou, Guohua Zhou
      Pages 155-166
  4. Reagents Preparation

  5. Pyrosequencing-Based Bio-Barcodes

    1. Front Matter
      Pages 215-215
    2. Xiaodan Zhang, Haiping Wu, Zhiyao Chen, Guohua Zhou, Hideki Kambara
      Pages 217-230
    3. Zhiyao Chen, Xiaodan Zhang, Xiqun Liu, Bingjie Zou, Haiping Wu, Qinxin Song et al.
      Pages 231-242
    4. Chao Liang, Yanan Chu, Sijia Cheng, Haiping Wu, Bingjie Zou, Qinxin Song et al.
      Pages 243-255
  6. Instrumentation

    1. Front Matter
      Pages 265-265
    2. Guohua Zhou, Masao Kamahori, Kazunori Okano, Kunio Harada, Hideki Kambara
      Pages 267-276
    3. Huan Huang, Haiping Wu, Pengfeng Xiao, Bingjie Zou, Qinxin Song, Guohua Zhou
      Pages 277-287
    4. Huan Huang, Pengfeng Xiao, Zongtai Qi, Bingjie Zou, Qinxin Song, Guohua Zhou
      Pages 289-300
  7. Clinical Practice

    1. Front Matter
      Pages 301-301
    2. Hui Ye, Haiping Wu, Huan Huang, Yunlong Liu, Bingjie Zou, Qinxin Song et al.
      Pages 303-313
    3. Qinxin Song, Hua Jing, Haiping Wu, Bingjie Zou, Guohua Zhou, Hideki Kambara
      Pages 315-325
    4. Hua Jing, Qinxin Song, Zhiyao Chen, Bingjie Zou, Guohua Zhou, Hideki Kambara
      Pages 327-338
    5. Huiyong Yang, Huan Huang, Haiping Wu, Bingjie Zou, Qinxin Song, Guohua Zhou et al.
      Pages 349-359
    6. Xiaodan Zhang, Haiping Wu, Zhiyao Chen, Bingjie Zou, Qinxin Song, Guohua Zhou
      Pages 361-369
    7. Weipeng Wang, Haiping Wu, Bingjie Zou, Qinxin Song, Guohua Zhou
      Pages 371-380
    8. Zheng Xiang, Yunlong Liu, Xiaoqing Xing, Bingjie Zou, Qinxin Song, Guohua Zhou
      Pages 381-389
    9. Qinxin Song, Huiyong Yang, Bingjie Zou, Hideki Kambara, Guohua Zhou
      Pages 391-398
  8. Back Matter
    Pages 399-407

About this book

Introduction

The aim of this book is to improve pyrosequencing protocols as well as instrumentation for better clinical use by describing improvements and novel applications of pyrosequencing technology.  Divided into five parts, the book’s thirty chapters explore advances in pyrosequencing template preparation, pyrosequencing technology innovations, multiplex pyrosequencing based on barcodes, the miniaturization of pyrosequencing equipment, as well as various applications. As part of the Springer Protocols program, chapters contain the kind of detail and practical implementation advice to guarantee successful results in the lab.

Comprehensive and thorough, Advances and Clinical Practice in Pyrosequencing serves as a valuable reference for researchers who are engaged in personalized medicine, disease control, and DNA diagnosis in numerous other fields.

Keywords

Gene expression Personalized medicine Pharmacogenomics Polymerase chain reaction Single nucleotide polymorphism Template preparation Multiplex sequencing Equipment miniaturization Pyrosequencer

Editors and affiliations

  • Guohua Zhou
    • 1
  • Qinxin Song
    • 2
  1. 1.State Key Laboratory of Analytical ChemiMedical School of Nanjing UniversityNanjingChina
  2. 2.Key Laboratory of Drug Quality ControlChina Pharmaceutical UniversityNanjingChina

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-3308-2
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3306-8
  • Online ISBN 978-1-4939-3308-2
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • Buy this book on publisher's site
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