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P-Type ATPases

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Maike Bublitz

    1. Department of Biochemistry, Biocenter, University of Oxford, Oxford, United Kingdom

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1377)

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Table of contents (43 protocols)

  1. Activity Assays

    1. Assay of Flippase Activity in Proteoliposomes Using Fluorescent Lipid Derivatives

      • Magdalena Marek, Thomas Günther-Pomorski
      Pages 181-191

  2. In Vitro Functional Studies

    1. Front Matter

      Pages 193-193
      Download chapter PDF

  3. In Vitro Functional Studies

    1. The Use of Metal Fluoride Compounds as Phosphate Analogs for Understanding the Structural Mechanism in P-type ATPases

      • Stefania J. Danko, Hiroshi Suzuki
      Pages 195-209

    2. Phosphorylation/Dephosphorylation Assays

      • Hiroshi Suzuki
      Pages 211-226

    3. Tryptophan Fluorescence Changes Related to Ca2+-ATPase Function

      • Pankaj Sehgal, Claus Olesen, Jesper V. Møller
      Pages 227-230

  4. Ligand Binding Studies

    1. Front Matter

      Pages 231-231
      Download chapter PDF

    2. Determination of the ATP Affinity of the Sarcoplasmic Reticulum Ca2+-ATPase by Competitive Inhibition of [γ-32P]TNP-8N3-ATP Photolabeling

      • Johannes D. Clausen, David B. McIntosh, David G. Woolley, Jens Peter Andersen
      Pages 233-259

    3. Ca2+ Binding and Transport Studied with Ca2+/EGTA Buffers and 45Ca2+

      • Pankaj Sehgal, Claus Olesen, Jesper V. Møller
      Pages 261-266

    4. Assay of Copper Transfer and Binding to P1B-ATPases

      • Teresita Padilla-Benavides, José M. Argüello
      Pages 267-277

  5. Electrophysiology

    1. Front Matter

      Pages 279-279
      Download chapter PDF

    2. Voltage Clamp Fluorometry of P-Type ATPases

      • Robert E. Dempski
      Pages 281-291

    3. Electrophysiological Measurements on Solid Supported Membranes

      • Francesco Tadini-Buoninsegni, Gianluca Bartolommei
      Pages 293-303

    4. Electrophysiological Characterization of Na,K-ATPases Expressed in Xenopus laevis Oocytes Using Two-Electrode Voltage Clamping

      • Florian Hilbers, Hanne Poulsen
      Pages 305-318

  6. Functional Studies by Cell Culture and Transgenic Animals

    1. Front Matter

      Pages 319-319
      Download chapter PDF

    2. Functional Studies of Na+,K+-ATPase Using Transfected Cell Cultures

      • Elena Arystarkhova, Kathleen J. Sweadner
      Pages 321-332

    3. HPLC Neurotransmitter Analysis

      • Thomas Hellesøe Holm, Toke Jost Isaksen, Karin Lykke-Hartmann
      Pages 333-340

    4. Behavior Test Relevant to α2/α3Na+/K+-ATPase Gene Modified Mouse Models

      • Toke Jost Isaksen, Thomas Hellesøe Holm, Karin Lykke-Hartmann
      Pages 341-351

    5. Zebrafish Whole-Mount In Situ Hybridization Followed by Sectioning

      • Canan Doganli, Jens Randel Nyengaard, Karin Lykke-Hartmann
      Pages 353-363

    6. Whole-Mount Immunohistochemistry for Anti-F59 in Zebrafish Embryos (1–5 Days Post Fertilization (dpf))

      • Canan Doganli, Lucas Bukata, Karin Lykke-Hartmann
      Pages 365-369

    7. Cell-Based Lipid Flippase Assay Employing Fluorescent Lipid Derivatives

      • Maria S. Jensen, Sara Costa, Thomas Günther-Pomorski, Rosa L. López-Marqués
      Pages 371-382
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Keywords

About this book

This volume provides a broad collection of protocols for many of the common experimental techniques used for the characterization of P-type ATPases.  P-Type ATPases: Methods and Protocols provides comprehensive practical instructions for all researchers in the P-type ATPase field, from the protein biochemist to the mouse geneticist, covering the identification and classification, isolation, purification, in vitro characterization, knock-out studies, as well as crystallization and structural analysis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, P-Type ATPases: Methods and Protocols aims to ensure successful results in the further study of this vital field of research.

Editors and Affiliations

  • Department of Biochemistry, Biocenter, University of Oxford, Oxford, United Kingdom

    Maike Bublitz

Bibliographic Information

  • Book Title: P-Type ATPases

  • Book Subtitle: Methods and Protocols

  • Editors: Maike Bublitz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3179-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3178-1Published: 23 December 2015

  • Softcover ISBN: 978-1-4939-7995-0Published: 29 March 2019

  • eBook ISBN: 978-1-4939-3179-8Published: 22 December 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 544

  • Number of Illustrations: 56 b/w illustrations, 51 illustrations in colour

  • Topics: Membrane Biology

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