Kinase Screening and Profiling

Methods and Protocols

  • Hicham Zegzouti
  • Said A. Goueli

Part of the Methods in Molecular Biology book series (MIMB, volume 1360)

Table of contents

  1. Front Matter
    Pages i-x
  2. Yong Jia, Mari Manuia, Jose Juarez
    Pages 1-18
  3. Krisna C. Duong-Ly, Jeffrey R. Peterson
    Pages 87-95
  4. Christophe Antczak, Hakim Djaballah
    Pages 97-106
  5. Silke M. Bauer, Jakub M. Kubiak, Ulrich Rothbauer, Stefan Laufer
    Pages 123-148
  6. Leanna R. Gentry, Andrei V. Karginov, Klaus M. Hahn, Channing J. Der
    Pages 157-167
  7. Shenshen Lai, Dirk F. H. Winkler, Hong Zhang, Steven Pelech
    Pages 183-202
  8. Back Matter
    Pages 217-223

About this book

Introduction

This volume focuses on screening and profiling kinase inhibitors or activators. Chapters in this book cover several facets of the drug discovery processes starting with target identification, and assay development and screening chemical libraries for hit identification and lead optimization. The profiling portion of Kinase Screening and Profiling: Methods and Protocols discusses several strategies and techniques that are required to minimize off target hits and reduce cellular toxicities that are caused by liability kinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Timely and authoritative, Kinase Screening and Profiling: Methods and Protocols, will benefit scientists and researchers who are interested in exploring the role of kinases in academic, as well as pharmaceutical research.

Keywords

ATP competitive inhibitors assay development cellular differentiation cellular growth cellular toxicities celular physiology enzymes hit identification target identification

Editors and affiliations

  • Hicham Zegzouti
    • 1
  • Said A. Goueli
    • 2
  1. 1.Promega CorporationMadisonUSA
  2. 2.Promega CorporationMadisonUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-3073-9
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3072-2
  • Online ISBN 978-1-4939-3073-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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