Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1338)
Access this book
Tax calculation will be finalised at checkout
Other ways to access
Table of contents (19 chapters)
Keywords
About this book
This volume provides a comprehensive collection of protocols on new technology across various model organisms. Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, TALENs: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Editors and Affiliations
Bibliographic Information
Book Title: TALENs
Book Subtitle: Methods and Protocols
Editors: Ralf Kühn, Wolfgang Wurst, Benedikt Wefers
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2932-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-2931-3Published: 07 October 2015
Softcover ISBN: 978-1-4939-4905-2Published: 23 August 2016
eBook ISBN: 978-1-4939-2932-0Published: 06 October 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 274
Number of Illustrations: 15 b/w illustrations, 50 illustrations in colour
Topics: Human Genetics, Animal Genetics and Genomics, Animal Models
Industry Sectors: Biotechnology, Pharma