Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1332)
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Table of contents (16 protocols)
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Front Matter
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VEGF and VEGF Receptors
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Front Matter
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Modulation and Analysis of VEGF Dependent Signaling
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Front Matter
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In Vivo Models to Study VEGF Signaling
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Front Matter
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About this book
This volume provides a collection of protocols for studying and manipulating VEGF signaling pathways in vitro and in vivo, and in particular, aims to present a range of both firmly established and newly emerging technologies, including those that are amenable to aiding in drug discovery or pre-clinical investigations. Each of the three sections begins with an introductory overview followed by supporting relevant methods. A major advantage of this book is that each chapter not only contains methodological detail rarely seen in other literature, but also contains a section on key notes and troubleshooting advice. Part I focuses on quantification of specific VEGF and VEGF receptor isoforms, beginning with a summary on VEGF splice variants, their function and their regulation. Part II relates to the study of VEGF dependent signaling in vitro, starting with a comprehensive overview of VEGF-mediated signal transduction in the cardiovascular system that highlights recent discoveries in the field. Part III first details genetic mouse models, then discusses techniques for studying physiological angiogenesis in the developing mouse embryo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, VEFG Signaling: Methods and Protocols is a useful tool for researching and understanding the basic biology of VEGF signaling and translating it into the clinic.
Editors and Affiliations
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Caversham, Reading, United Kingdom
Lorna Fiedler
About the editor
Lorna R. Fielder, Program in cardiovascular and Metabolic Disorders, Duke-NUS, Graduate Medical School Singapore, 8 College Road, Singapore 169857, Telephone: +65-6516-7666, Email: lorna.fiedler@gmail.com or l.fiedler@imperial.ac.uk.
Bibliographic Information
Book Title: VEGF Signaling
Book Subtitle: Methods and Protocols
Editors: Lorna Fiedler
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2917-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2916-0Published: 20 August 2015
Softcover ISBN: 978-1-4939-4370-8Published: 22 October 2016
eBook ISBN: 978-1-4939-2917-7Published: 18 August 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 225
Number of Illustrations: 13 b/w illustrations, 23 illustrations in colour
Topics: Biochemistry, general
Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma