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Human Embryonic Stem Cell Protocols

Editors:

  • Includes cutting-edge methods and protocols involving human embryonic stem cells
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1307)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xiv
  2. Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Blastocysts

    • Cara K. Bradley, Julia Schaft, Tammie K. Roy, Biljana Dumevska, Teija T. Peura
    Pages 1-23
  3. Characterizing Pluripotent Stem Cells Using the TaqMan® hPSC ScorecardTM Panel

    • Jeffrey Fergus, Rene Quintanilla, Uma Lakshmipathy
    Pages 25-37
  4. Growth of Human Pluripotent Stem Cells Using Functional Human Extracellular Matrix

    • Andres Sanz-Garcia, Miodrag Stojkovic, Carmen Escobedo-Lucea
    Pages 39-60
  5. Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR

    • Amparo Galán, Carlos Simón
    Pages 89-104
  6. Study of Gap Junctions in Human Embryonic Stem Cells

    • Alice Pébay, Raymond C. B. Wong
    Pages 105-121
  7. Immunofluorescence Microscopy and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Including Primary Cilia Associated Signaling Pathways

    • Maj Linea Vestergaard, Aashir Awan, Caroline Becker Warzecha, Søren Tvorup Christensen, Claus Yding Andersen
    Pages 123-140
  8. Analysis of Intracellular Calcium Signaling in Human Embryonic Stem Cells

    • Adrienn Péntek, Katalin Pászty, Ágota Apáti
    Pages 141-147
  9. Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 System

    • Haipeng Xue, Jianbo Wu, Shenglan Li, Mahendra S. Rao, Ying Liu
    Pages 173-190
  10. Differentiation of Human Embryonic Stem Cells on Periodontal Ligament Fibroblasts

    • Y. Murat Elçin, Bülend İnanç, A. Eser Elçin
    Pages 223-235
  11. Human Embryonic and Hepatic Stem Cell Differentiation Visualized in Two and Three Dimensions Based on Serial Sections

    • Peter S. Vestentoft, Christian B. Brøchner, Niels Lynnerup, Claus Yding Andersen, Kjeld Møllgård
    Pages 245-262
  12. Microgrooved Surface Modulates Neuron Differentiation in Human Embryonic Stem Cells

    • David Lu, Chi-Shuo Chen, Chao-Sung Lai, Sushant Soni, Taranze Lam, Clarence Le et al.
    Pages 281-287

About this book

The potential of human embryonic stem cells to advance not only regenerative medicine applications but also our fundamental understanding of stem cell biology continues to drive interest in research with these cells. This detailed volume collects some of the most interesting and useful protocols that have emerged in the area over the last several years. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.

Thorough and practical, Human Embryonic Stem Cell Protocols, Third Edition serves as a valuable resource to all those interested in exploring stem cell biology questions in a research setting.

Editors and Affiliations

  • Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Human Embryonic Stem Cell Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2668-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-2667-1Published: 11 August 2015

  • Softcover ISBN: 978-1-4939-4998-4Published: 25 October 2016

  • eBook ISBN: 978-1-4939-2668-8Published: 10 August 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XIV, 399

  • Number of Illustrations: 28 b/w illustrations, 40 illustrations in colour

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access