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Small Non-Coding RNAs

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Mathieu Rederstorff

    1. Université de Lorraine, Vandoeuvre-lès-Nancy, France

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1296)

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Table of contents (21 protocols)

  1. Small Non-coding RNAs Applications

    1. RLM-RACE, PPM-RACE, and qRT-PCR: An Integrated Strategy to Accurately Validate miRNA Target Genes

      • Chen Wang, Jinggui Fang
      Pages 175-186

    2. Dual Luciferase Gene Reporter Assays to Study miRNA Function

      • Thomas Clément, Véronique Salone, Mathieu Rederstorff
      Pages 187-198

    3. Gene Expression Knockdown by Transfection of siRNAs into Mammalian Cells

      • Yoann Abel, Mathieu Rederstorff
      Pages 199-202

    4. Efficient and Selective Knockdown of Small Non-Coding RNAs

      • Xue-Hai Liang, Wen Shen, Stanley T. Crooke
      Pages 203-211

    5. Cell-SELEX: In Vitro Selection of Synthetic Small Specific Ligands

      • Helena Dickinson, Melanie Lukasser, Günter Mayer, Alexander Hüttenhofer
      Pages 213-224

    6. Small Non-Coding RNAs and Aptamers in Diagnostics and Therapeutics

      • Marissa Leonard, Yijuan Zhang, Xiaoting Zhang
      Pages 225-233

  2. Back Matter

    Pages 235-238
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Keywords

About this book

This volume contains state-of-the-art methods tackling all aspects of small non-coding RNAs biology.  Small Non-Coding RNAs: Methods and Protocols guides readers through customized dedicated protocols and technologies that will be of valuable help to all those willing to contribute deciphering the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubles troubleshooting and avoiding known pitfalls.

Instructive and practical, Small Non-Coding RNAs: Methods and Protocols reaches out to biochemists, cellular and molecular biologists already working in the field of RNA biology  and to those just starting to study small non-coding RNAs.

Editors and Affiliations

  • Université de Lorraine, Vandoeuvre-lès-Nancy, France

    Mathieu Rederstorff

About the editor

Mathieu Rederstorff, PhD Associate Professor Laboratoire IMoPA UMR 7365 CNRS/UL Equipe ARN, RNP - Maturation, Structure, Fonction - Université de Lorraine Biopôle/Campus Médecine Brabois 9 Avenue de la Forêt de Haye BP 184 54506 Vandoeuvre-lès-Nancy FRANCE Tel: +33 3 83 68 55 31 (office) +33 3 83 68 55 25 (lab) +33 6 49 12 67 11 (mobile) Fax: +33 3 83 68 55 09 (fax) mathieu.rederstorff@univ-lorraine.fr mathieu.rederstorff@maem.uhp-nancy.fr

Bibliographic Information

  • Book Title: Small Non-Coding RNAs

  • Book Subtitle: Methods and Protocols

  • Editors: Mathieu Rederstorff

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2547-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2546-9Published: 21 March 2015

  • Softcover ISBN: 978-1-4939-4903-8Published: 05 October 2016

  • eBook ISBN: 978-1-4939-2547-6Published: 19 March 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 238

  • Number of Illustrations: 20 b/w illustrations, 15 illustrations in colour

  • Topics: Human Genetics

  • Industry Sectors: Biotechnology, Pharma

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