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  • © 2015

Mammary Stem Cells

Methods and Protocols

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1293)

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-2519-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 139.99
Price excludes VAT (USA)
Hardcover Book USD 169.99
Price excludes VAT (USA)

This is a preview of subscription content, access via your institution.

Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
  2. Breast Cancer Stem Cells: Current Advances and Clinical Implications

    • Ming Luo, Shawn G. Clouthier, Yadwinder Deol, Suling Liu, Sunitha Nagrath, Ebrahim Azizi et al.
    Pages 1-49
  3. FACS Sorting Mammary Stem Cells

    • Oihana Iriondo, Miriam Rábano, María d. M. Vivanco
    Pages 63-72
  4. Side Population

    • Fariba Behbod, Maria d. M. Vivanco
    Pages 73-81
  5. Single-Cell Genome and Transcriptome Processing Prior to High-Throughput Sequencing

    • Ana M. Aransay, Laura Barcena, Aintzane Gonzalez-Lahera, Nuria Macias-Camara
    Pages 83-114
  6. Shotgun Proteomics on Tissue Specimens Extracted with Acid Guanidinium-Thiocyanate-Phenol-Chloroform

    • René B. H. Braakman, Anieta M. Sieuwerts, Arzu Umar
    Pages 115-122
  7. Antibody-Based Capture of Target Peptides in Multiple Reaction Monitoring Experiments

    • Tommaso De Marchi, Eric Kuhn, Steven A. Carr, Arzu Umar
    Pages 123-135
  8. Lentiviral Transduction of Mammary Epithelial Cells

    • Richard Iggo, Elodie Richard
    Pages 137-160
  9. The Transplantation of Mouse Mammary Epithelial Cells into Cleared Mammary Fat Pads

    • Marisa M. Faraldo, Marina A. Glukhova, Marie-Ange Deugnier
    Pages 161-172
  10. Humanization of the Mouse Mammary Gland

    • A. Wronski, L. M. Arendt, Charlotte Kuperwasser
    Pages 173-186
  11. Mammary Cancer Stem Cells Reinitiation Assessment at the Metastatic Niche: The Lung and Bone

    • Marc Guiu, Enrique J. Arenas, Sylwia Gawrzak, Milica Pavlovic, Roger R. Gomis
    Pages 221-229
  12. Mammary Stem Cells: A Clinician’s View

    • José Schneider
    Pages 267-271
  13. Back Matter

    Pages 273-275

About this book

The identification of normal and breast cancer stem cells has offered a new vision of this heterogeneous disease and new hopes for its prognosis and treatment. This volume provides an overview of recent developments in mammary stem cell research and discusses the many varieties of approaches used by researchers to investigate the properties and functions of mammary stem cells. The beginning chapters provide readers with an introduction to mammary stem cells, and the processes used to characterize stem cells and isolate them via fluorescent activated cell sorting. The next few chapters discuss DNA and mRNA sequencing, proteomic techniques to help profile cells, lentiviral cell transduction for gene expression, and in vivo lineage tracing. The final few chapters are dedicated to following stem cells from their initial niche to the new microenvironment at their metastasis site, and to studying these cells using physical and mathematical approaches. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Authoritative and cutting-edge, Mammary Stem Cells: Methods and Protocols aims to help members of the scientific community explore the behavior of stem cells and how to work with them in order to guide the design of new and complimentary strategies to be applied in the clinic with the ultimate end goal of fighting breast cancer.

Keywords

  • breast cancer
  • embryogenesis
  • fluorescent activated cell sorting
  • gene expression
  • in vivo lineage tracing
  • mammary gland
  • metastasis
  • regenerative medicine
  • stem cells

Editors and Affiliations

  • CIC bioGUNE, Technological Park of Bizkaia, Derio, Spain

    Maria del Mar Vivanco

About the editor

María dM Vivanco, PhD CIC bioGUNE Cell Biology & Stem Cells Unit Parque Tecnológico de Bizkaia, Ed 801A 48160 Derio (Bizkaia) Spain Tel: +34 94 406 1322 email: mdmvivanco@cicbiogune.es

Bibliographic Information

  • Book Title: Mammary Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Maria del Mar Vivanco

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2519-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2518-6

  • Softcover ISBN: 978-1-4939-4849-9

  • eBook ISBN: 978-1-4939-2519-3

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 275

  • Number of Illustrations: 13 b/w illustrations, 45 illustrations in colour

  • Topics: Stem Cell Biology

  • Industry Sectors: Biotechnology, Pharma

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-2519-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 139.99
Price excludes VAT (USA)
Hardcover Book USD 169.99
Price excludes VAT (USA)