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  • © 2015

Mycobacteria Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1285)

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Table of contents (25 protocols)

  1. Macrophage Infection Models for Mycobacterium tuberculosis

    • Benjamin K. Johnson, Robert B. Abramovitch
    Pages 329-341
  2. Infection of Human Neutrophils to Study Virulence Properties of Mycobacterium tuberculosis

    • Tobias Dallenga, Björn Corleis, Ulrich E. Schaible
    Pages 343-355
  3. Isolation of Bead Phagosomes to Study Virulence Function of M. tuberculosis Cell Wall Lipids

    • Anna C. Geffken, Emmanuel C. Patin, Ulrich E. Schaible
    Pages 357-368
  4. Live Imaging of Mycobacterium marinum Infection in Dictyostelium discoideum

    • Caroline Barisch, Ana T. López-Jiménez, Thierry Soldati
    Pages 369-385
  5. Testing Chemical and Genetic Modulators in Mycobacterium tuberculosis Infected Cells Using Phenotypic Assays

    • Vincent Delorme, Ok-Ryul Song, Alain Baulard, Priscille Brodin
    Pages 387-411
  6. Erratum to: Genetic Dissection of Mycobacterial Biofilms

    • Anil K. Ojha, William R. Jacobs Jr., Graham F. Hatfull
    Pages E1-E1
  7. Back Matter

    Pages 413-416

About this book

Updated and revised, this thorough volume provides a selection of the newest methods, as well as some of the basic methods required for a mycobacterial research laboratory. Mycobacteria Protocols, Third Edition guides readers through fractionation and analysis of macromolecules, from nucleic acids to proteins, complex lipids, and metabolites. Detailed and comprehensive protocols are provided for protein and lipid/glycolipid analysis using well-established methods; these are now complemented by a metabolomics chapter in which the complement of metabolites can be profiled. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Mycobacteria Protocols, Third Edition will be a resource both to those working in the field and to newcomers.

Editors and Affiliations

  • Infectious Disease Research Inst (IDRI), Seattle, USA

    Tanya Parish, David M. Roberts

Bibliographic Information

  • Book Title: Mycobacteria Protocols

  • Editors: Tanya Parish, David M. Roberts

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2450-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2449-3Published: 17 March 2015

  • Softcover ISBN: 978-1-4939-5582-4Published: 05 October 2016

  • eBook ISBN: 978-1-4939-2450-9Published: 16 March 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XII, 416

  • Number of Illustrations: 51 b/w illustrations, 37 illustrations in colour

  • Topics: Microbiology

  • Industry Sectors: Biotechnology, Chemical Manufacturing, Consumer Packaged Goods, Pharma

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access