Chromosomal Mutagenesis

  • Shondra M.¬†Pruett-Miller

Part of the Methods in Molecular Biology book series (MIMB, volume 1239)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Stephen J. Pettitt, E-Pien Tan, Kosuke Yusa
    Pages 15-28
  3. Rob Howes, Christine Schofield
    Pages 75-103
  4. Tomas Cermak, Colby G. Starker, Daniel F. Voytas
    Pages 133-159
  5. Jonathan L. Schmid-Burgk, Tobias Schmidt, Veit Hornung
    Pages 161-169
  6. Le Cong, Feng Zhang
    Pages 197-217
  7. John Fetter, Andrey Samsonov, Nathan Zenser, Fan Zhang, Hongyi Zhang, Dmitry Malkov
    Pages 231-240
  8. Fuqiang Chen, Shondra M. Pruett-Miller, Gregory D. Davis
    Pages 251-265
  9. Victoria M. Bedell, Stephen C. Ekker
    Pages 291-305

About this book

Introduction

This new edition explores current and emerging mutagenesis methods focusing specifically on mammalian systems and commonly used model organisms through comprehensive coverage and detailed protocols. Since the first edition, major advances and discoveries have made chromosomal mutagenesis a widely used technique and one that is available to any molecular biology laboratory, and this collection provides detailed protocols, case-studies, and reviews from thought-leaders in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and fully updated, Chromosomal Mutagenesis, Second Edition aims to help speed scientific discovery and aid in the next advances in the field.

Keywords

Genome editing tools Genome engineering Homologous recombination Mammalian systems Model organisms Mutagenesis methods Nuclease modification

Editors and affiliations

  • Shondra M.¬†Pruett-Miller
    • 1
  1. 1.Department of Genetics, Genome Engineering and iPSC CenterWashington University School of MedicineSt. LouisUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-1862-1
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1861-4
  • Online ISBN 978-1-4939-1862-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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