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The Nucleus

  • Ronald Hancock

Part of the Methods in Molecular Biology book series (MIMB, volume 1228)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Isolation of Nuclei

    1. Front Matter
      Pages 1-1
    2. Karl Katholnig, Marko Poglitsch, Markus Hengstschläger, Thomas Weichhart
      Pages 15-23
  3. Nucleoli

    1. Front Matter
      Pages 33-33
    2. Zhou Fang Li, Yun Wah Lam
      Pages 35-42
  4. Genes and Chromatin

    1. Front Matter
      Pages 53-53
    2. Stephanie D. Byrum, Sean D. Taverna, Alan J. Tackett
      Pages 83-92
    3. Christopher R. Brown, Julian A. Eskin, Stephan Hamperl, Joachim Griesenbeck, Melissa S. Jurica, Hinrich Boeger
      Pages 93-121
    4. Huzefa Dungrawala, David Cortez
      Pages 123-131
    5. Alexandre David, Jonathan W. Yewdell
      Pages 133-142
  5. The Intranuclear Milieu

    1. Front Matter
      Pages 143-143
    2. Frédérique Nolin, Dominique Ploton, Laurence Wortham, Pavel Tchelidze, Hélène Bobichon, Vincent Banchet et al.
      Pages 145-158
    3. Agata Banach-Latapy, Michèle Dardalhon, Meng-Er Huang
      Pages 159-169
  6. Imaging Nuclear Structures

    1. Front Matter
      Pages 171-171
    2. Florian Arnhold, Andrea Scharf, Anna von Mikecz
      Pages 187-202
    3. Jean Ollion, Julien Cochennec, François Loll, Christophe Escudé, Thomas Boudier
      Pages 203-222
    4. Sandeep Chakraborty, Ishita Mehta, Mugdha Kulashreshtha, B. J. Rao
      Pages 223-233
  7. Back Matter
    Pages 235-237

About this book

Introduction

This volume presents detailed, recently-developed protocols ranging from isolation of nuclei to purification of chromatin regions containing single genes, with a particular focus on some less well-explored aspects of the nucleus. The methods described include new strategies for isolation of nuclei, for purification of cell type-specific nuclei from a mixture, and for rapid isolation and fractionation of nucleoli. For gene delivery into and expression in nuclei, a novel gentle approach using gold nanowires is presented. As the concentration and localization of water and ions are crucial for macromolecular interactions in the nucleus, a new approach to measure these parameters by correlative optical and cryo-electron microscopy is described. The Nucleus, Second Edition presents methods and software for high-throughput quantitative analysis of 3D fluorescence microscopy images, for quantification of the formation of amyloid fibrils in the nucleus, and for quantitative analysis of chromosome territory localization. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, The Nucleus, Second Edition seeks to serve both professionals and novices with its well-honed methods for the study of the nucleus.

Keywords

cell nucleus intranuclear milieu nuclei isolation nucleolus proteomic analysis

Editors and affiliations

  • Ronald Hancock
    • 1
  1. 1.Laval University Cancer Research CenterQuébecCanada

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-1680-1
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1679-5
  • Online ISBN 978-1-4939-1680-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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