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Cytotoxic T-Cells

Methods and Protocols

  • Elena Ranieri

Part of the Methods in Molecular Biology book series (MIMB, volume 1186)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Margherita Gigante, Elena Ranieri
    Pages 13-20
  3. Iulia Popescu, Matthew Pipeling, Jason Akulian, John McDyer
    Pages 21-47
  4. Sharon Natasha Cox
    Pages 49-63
  5. Devin B. Lowe, Jennifer L. Taylor, Walter J. Storkus
    Pages 65-74
  6. Elena Ranieri, Iulia Popescu, Margherita Gigante
    Pages 75-86
  7. Angela Montecalvo, Adriana T. Larregina, Adrian E. Morelli
    Pages 87-102
  8. Giuseppe Stefano Netti
    Pages 103-119
  9. Gaetano Faleo, Per-Olof Berggren, Antonello Pileggi
    Pages 121-129
  10. Matteo Accetturo, Paola Pontrelli, Loreto Gesualdo
    Pages 131-199
  11. Paola Pontrelli, Matteo Accetturo, Loreto Gesualdo
    Pages 201-232
  12. Massimo Papale, Maria Teresa Rocchetti
    Pages 233-242
  13. Maria Teresa Rocchetti, Massimo Papale, Loreto Gesualdo
    Pages 243-251
  14. Maddalena Gigante, Sterpeta Diella, Elena Ranieri
    Pages 253-268
  15. Giacomina Brunetti, Angela Oranger, Silvia Colucci, Maria Grano
    Pages 269-281
  16. Federica Rascio, Chiara Divella, Giuseppe Grandaliano
    Pages 283-294
  17. Back Matter
    Pages 295-301

About this book

Introduction

 In Cytotoxic T-Cells: Methods and Protocols, leading experts in the field provide a collection of state-of-the art methods and protocols encompassing a wide array of systems biology approaches for Cytotoxic T-Cell research. Dived into three main sections, the first part of the volume analyzes the isolation of T Cells along with their expansion and characterization according to different methods. The second part describes required techniques for intracellular signaling, monitoring of antigen T cell specific responses, CTL exosomes, and microscopy and in vivo imaging applied to CTL studies.  The final section focuses on specialist applications of molecular methods into the study of CTL, including next generation sequencing of the Jack/stat pathway and CTL involvement in bone remodeling and transplantation.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Cytotoxic T-Cells: Methods and Protocols seeks to aid scientists in the further study into concepts of laboratory methods using systems biology.

Keywords

CTL exosomes CTL studies Cytotoxic T-Cells Jack/stat pathway Omics approaches antigen T cell specific responses bone remodeling bone transplantation in vivo imaging intracellular signaling isolation of T cells molecular methods

Editors and affiliations

  • Elena Ranieri
    • 1
  1. 1.Department of Medical and Surgical SciencesUniversity of FoggiaFoggiaItaly

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-1158-5
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1157-8
  • Online ISBN 978-1-4939-1158-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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