RNA Mapping

Methods and Protocols

  • M. Lucrecia Alvarez
  • Mahtab Nourbakhsh

Part of the Methods in Molecular Biology book series (MIMB, volume 1182)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Structural RNA Mapping

    1. Front Matter
      Pages 1-1
    2. Marc R. Reboll, M. Lucrecia Alvarez, Mahtab Nourbakhsh
      Pages 3-18
    3. Victoria Zismann, Mahtab Nourbakhsh
      Pages 19-25
    4. Mahtab Nourbakhsh
      Pages 35-42
    5. Steven G. Chaulk, Richard P. Fahlman
      Pages 43-55
    6. Stefania Cotta Doné, Olga Beltcheva
      Pages 57-71
    7. Susanna Cirera, Peter K. Busk
      Pages 73-81
    8. Kasandra L. Burgos, Kendall Van Keuren-Jensen
      Pages 83-92
    9. Adrienn Gyongyosi, Otto Docs, Zsolt Czimmerer, Laszlo Orosz, Attila Horvath, Olga Török et al.
      Pages 105-119
    10. Marina C. Costa, Ana Lúcia Leitão, Francisco J. Enguita
      Pages 121-129
  3. Functional RNA Mapping

    1. Front Matter
      Pages 171-171
    2. Marc R. Reboll, Mahtab Nourbakhsh
      Pages 173-178
    3. Sarah Mehrtens, Marc R. Reboll
      Pages 179-185
    4. Marc R. Reboll
      Pages 187-194
    5. Birgit Ritter, Marc R. Reboll
      Pages 195-201

About this book


In RNA Mapping- Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study RNA. These include protocols for the consequence of the emerging interest in the characterization of cellular RNAs urged by their potential use as diagnostic biomarkers or therapeutic targets. In particular, the biological relevance of microRNAs in human physiology and disease development is highlighted in the 16 chapters focused on methods for their physical and functional mapping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, RNA Mapping- Methods and Protocols provides instruction and inspiration for scientists who are facing the challenges of the discovery and/or functional characterization of RNA molecules for a wide variety of applications ranging from novel biomedical diagnostics to therapeutics and biomaterials.


RNA boundaries RNA identification RNA profiling cellular RNAs diagnostic biomarkers mapping of internal ribosome microRNA targets prediction novel RNA functions quantitative real-time PCR regulatory mRNA elements target RNAs tertiary RNA therapeutic targets

Editors and affiliations

  • M. Lucrecia Alvarez
    • 1
  • Mahtab Nourbakhsh
    • 2
  1. 1.Diabetes, Cardiovascular, and Metabolic DiseasesTranslational Genomics Research InstitutePhoenixUSA
  2. 2.Department of Plastic SurgeryUniversity Hospital of the RWTH AachenGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-1062-5
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1061-8
  • Online ISBN 978-1-4939-1062-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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