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  • Book
  • © 2014

Pseudomonas Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1149)

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Table of contents (61 protocols)

  1. Proteins and Protein Transport

    1. Membrane Proteomics of Pseudomonas aeruginosa

      • Zofia Magnowska, Isabel Hartmann, Lothar Jänsch, Dieter Jahn
      Pages 213-224
  2. Small Molecules and Polysaccharides

    1. Front Matter

      Pages 243-243
    2. Biosensors for Qualitative and Semiquantitative Analysis of Quorum Sensing Signal Molecules

      • Matthew Fletcher, Miguel Cámara, David A. Barrett, Paul Williams
      Pages 245-254
    3. LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules

      • Catharine A. Ortori, Nigel Halliday, Miguel Cámara, Paul Williams, David A. Barrett
      Pages 255-270
    4. Metabolic Footprinting: Extracellular Metabolomic Analysis

      • Volker Behrends, Huw D. Williams, Jacob G. Bundy
      Pages 281-292
    5. Pyoverdine and Pyochelin Measurements

      • Françoise Hoegy, Gaetan L. A. Mislin, Isabelle J. Schalk
      Pages 293-301
    6. Measurement of Phenazines in Bacterial Cultures

      • Suzanne E. Kern, Dianne K. Newman
      Pages 303-310
    7. Extraction and Measurement of NAD(P)+ and NAD(P)H

      • Suzanne E. Kern, Alexa Price-Whelan, Dianne K. Newman
      Pages 311-323
    8. Cyanide Measurements in Bacterial Culture and Sputum

      • Chandrika Goh Nair, Ben Ryall, Huw D. Williams
      Pages 325-336
    9. Monitoring Iron Uptake by Siderophores

      • Françoise Hoegy, Isabelle J. Schalk
      Pages 337-346
    10. Exopolysaccharide Quantification

      • Irina Sadovskaya
      Pages 347-357
    11. Liquid Chromatography/Mass Spectrometry for the Identification and Quantification of Rhamnolipids

      • Ahmad Mohammad Abdel-Mawgoud, François Lépine, Eric Déziel
      Pages 359-373
    12. LPS Quantitation Procedures

      • Joseph S. Lam, Erin M. Anderson, Youai Hao
      Pages 375-402
    13. Monitoring Lectin Interactions with Carbohydrates

      • Sophie de Bentzmann, Annabelle Varrot, Anne Imberty
      Pages 403-414
  3. Pseudomonas Genes and Gene Expression

    1. Front Matter

      Pages 415-415
    2. Mining the Pseudomonas Genome

      • Geoffrey L. Winsor, Fiona S. L. Brinkman
      Pages 417-432
    3. Identification of Bacterial Small RNAs by RNA Sequencing

      • María Gómez-Lozano, Rasmus Lykke Marvig, Søren Molin, Katherine S. Long
      Pages 433-456
    4. Gene Amplification and qRT-PCR

      • Cerith Jones, Alain Filloux
      Pages 457-468

About this book

In Pseudomonas aeruginosa, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been developed over the last decade. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Pseudomonas aeruginosa aids in the continuing study of new and cutting edge findings.

Editors and Affiliations

  • MRC Centre for Molecular Bacteriology and Infection (CMBI) Department of Life Sciences, Imperial College, London, UK

    Alain Filloux

  • Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain

    Juan-Luis Ramos

Bibliographic Information

  • Book Title: Pseudomonas Methods and Protocols

  • Editors: Alain Filloux, Juan-Luis Ramos

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0473-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0472-3Published: 13 May 2014

  • Softcover ISBN: 978-1-4939-4416-3Published: 03 September 2016

  • eBook ISBN: 978-1-4939-0473-0Published: 12 May 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVIII, 816

  • Number of Illustrations: 68 b/w illustrations, 89 illustrations in colour

  • Topics: Microbiology, Bacteriology

  • Industry Sectors: Biotechnology, Chemical Manufacturing, Consumer Packaged Goods, Pharma

Buy it now

Buying options

eBook USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 299.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access