Skip to main content
SpringerLink
Log in
Book cover

Handbook of Biological Confocal Microscopy

  • Book
  • © 1995

Overview

Editors:
  1. James B. Pawley

    1. Zoology Department, University of Wisconsin-Madison, Madison, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 74.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (37 chapters)

  1. Lens Aberrations in Confocal Fluorescence Microscopy

    • Stefan W. Hell, Ernst H. K. Stelzer
    Pages 347-354

  2. Real-Time Stereo (3D) Confocal Microscopy

    • G. J. Brakenhoff, K. Visscher
    Pages 355-362

  3. Signal-to-Noise in Confocal Microscopes

    • Colin J. R. Sheppard, Xiaosong Gan, Min Gu, Maitreyee Roy
    Pages 363-371

  4. Comparison of Wide-Field/Deconvolution and Confocal Microscopy for 3D Imaging

    • Peter J. Shaw
    Pages 373-387

  5. Light Microscopic Images Reconstructed by Maximum Likelihood Deconvolution

    • Timothy J. Holmes, Santosh Bhattacharyya, Joshua A. Cooper, David Hanzel, Vijaykumar Krishnamurthi, Wen-chieh Lin et al.
    Pages 389-402

  6. Direct View Confocal Imaging Systems Using a Slit Aperture

    • W. B. Amos, J. G. White
    Pages 403-415

  7. Two New High-Resolution Confocal Fluorescence Microscopies (4Pi, Theta) with One- and Two-Photon Excitation

    • Steffen Lindek, Ernst H. K. Stelzer, Stefan W. Hell
    Pages 417-430

  8. Optical Considerations at Ultraviolet Wavelengths in Confocal Microscopy

    • A. Christyne Bliton, James D. Lechleiter
    Pages 431-444

  9. Two-Photon Molecular Excitation in Laser-Scanning Microscopy

    • Winfried Denk, David W. Piston, Watt W. Webb
    Pages 445-458

  10. Video-Rate Confocal Microscopy

    • Roger Y. Tsien, Brian J. Bacskai
    Pages 459-478

  11. Confocal Microscopy with Transmitted Light

    • A. E. Dixon, Carol Cogswell
    Pages 479-490

  12. Fluorescence Lifetime Imaging, a New Tool in Confocal Microscopy

    • A. Draaijer, R. Sanders, H. C. Gerritsen
    Pages 491-505

  13. Imaging Immunogold Labels with Confocal Microscopy

    • Carol J. Cogswell
    Pages 507-513

  14. Fiberoptics in Confocal Microscopy

    • P. M. Delaney, M. R. Harris
    Pages 515-523

  15. Comparison of Various Optical Sectioning Methods

    • Charles J. Koester
    Pages 525-534

  16. Mass Storage and Hard Copy

    • Guy Cox
    Pages 535-548

  17. Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen

    • Victoria Centonze, James Pawley
    Pages 549-569

  18. Bibliography of Confocal Microscopes

    • Robert H. Webb
    Pages 571-577

  19. Back Matter

    Pages 579-632
    Download chapter PDF
Back to top

Keywords

About this book

This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.

Reviews

From the reviews of the third edition:

`Considerably augmented for [the] second edition...[contains] a vast amount of information....it will surely be much used.' Ultramicroscopy

"The audience for this book is primarily seniors and graduate students, core service operators, collaborating scientists, such as molecular biologists, physiologists, and clinicians who wish to complement and supplement their experience with sophisticated and quantitative microscopy. … The book is readable and instructive. … There are hundreds of diagrams, tables, and formulas, as well as hundreds of color images of the cells, tissues, organelles, and systems one can study with CFM. … The references are up to date, complete, and easy to find." (James B. Pawley, Veterinary Pathology, Vol. 45 (1), 2008)

Editors and Affiliations

  • Zoology Department, University of Wisconsin-Madison, Madison, USA

    James B. Pawley

Bibliographic Information

Publish with us

Policies and ethics

Back to top

Search

Navigation