Molecular Diagnostics

1. Front Matter

   Pages i-x

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2. Basic Molecular Biology

   1. Front Matter

      Pages 1-1

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   2. A Historical Perspective on the Clinical Diagnostic Laboratory

      • Robert E. Moore

      Pages 3-13

   3. An Overview of Nucleic Acid Chemistry, Structure, and Function

      • William B. Coleman

      Pages 15-33

   4. Basic Nucleic Acid Procedures

      • R. Keith Esch

      Pages 35-60

3. Molecular Technologies

   1. Front Matter

      Pages 61-61

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   2. Nucleic Acid Blotting Techniques

      • Sharon Collins Presnell

      Pages 63-87

   3. DNA Amplification Techniques

      • David H. Sorscher

      Pages 89-101

   4. RT-PCR

      • Robert B. Sisk

      Pages 103-121

   5. PCR-Based Methods for Mutation Detection

      • Elizabeth M. Rohlfs, W. Edward Highsmith Jr.

      Pages 123-162

   6. Nucleic Acid Hybridization and Amplification In Situ

      • Matthew S. Cowlen

      Pages 163-191

   7. Gene Therapy

      • Jeffrey S. Bartlett

      Pages 193-214

4. Applications to Molecular Pathology

   1. Front Matter

      Pages 215-215

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   2. An Overview of Molecular Genetics

      • Gregory J. Tsongalis

      Pages 217-228

   3. Genetic Basis of Neurologic and Neuromuscular Diseases

      • Myra J. Wick, Pamela A. Crifasi, Zhenyuan Wang, Stephen N. Thibodeau

      Pages 229-248

   4. Molecular Mechanisms of Endocrine Disorders

      • Bruce F. Bower, Carl D. Malchoff

      Pages 249-269

   5. Molecular Pathogenesis of Cardiovascular Disease

      • John H. Contois, Juch Chin Huang

      Pages 271-291

   6. Molecular Mutations in Human Neoplastic Disease

      • William B. Coleman, Gregory J. Tsongalis

      Pages 293-316

   7. Molecular Genetics and the Diagnosis of Hematological Malignancies

      • William N. Rezuke, Evelyn C. Abernathy

      Pages 317-339

   8. Molecular Techniques in Laboratory Diagnosis of Infectious Diseases

      • Jaber Aslanzadeh

      Pages 341-360

Notable practitioners describe how laboratory medicine is practiced today and illuminate how it will function tomorrow as the revolutionary advances afforded by molecular diagnostics become increasingly central to effective analysis. Proceeding from a discussion of elementary nucleic acid technology to a review of the more advanced techniques, the distinguished contributors lay the groundwork for a comprehensive understanding of their applications throughout clinical medicine. The result is a detailed description of those molecular technologies currently used in diagnostic laboratories, as well as those that seem particularly promising. Detailed discussions of specific clinical applications include those for cancer, hematological malignancies, cardiovascular disease, and neuromuscular, endocrine, and infectious diseases.

• DNA
• PCR
• biology
• cancer
• diagnostics
• gene expression
• gene therapy
• genes
• genetics
• infectious diseases
• molecular biology
• molecular genetics
• molecular mechanisms
• mutation
• nucleic acid

". . .highly recommended. . .for those interested in clinical molecular diagnosis."-American Journal of Clinical Pathology
". . .a very good introduction to the basic elements of molecular pathology. . .a clear, enjoyable read. . .an excellent discussion of methods."-Clinical Chemistry

• Department of Pathology and Laboratory Medicine, University of North Carolina School of Medicine, Chapel Hill, USA

  William B. Coleman

• Department of Pathology and Laboratory Medicine, Hartford Hospital, Hartford, USA

  Gregory J. Tsongalis

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