Metastasis Research Protocols

  • Miriam Dwek
  • Udo Schumacher
  • Susan A. Brooks

Part of the Methods in Molecular Biology book series (MIMB, volume 1070)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Olivier De Wever, An Hendrix, Astrid De Boeck, Frank Eertmans, Wendy Westbroek, Geert Braems et al.
    Pages 13-35
  3. Clara M. Yates, Helen M. McGettrick, Gerard B. Nash, G. Ed Rainger
    Pages 57-75
  4. Delphine Debruyne, Tom Boterberg, Marc E. Bracke
    Pages 77-92
  5. Marc E. Bracke, Virinder S. Parmar, Anthony L. DePass, Christian V. Stevens, Barbara W. Vanhoecke, Marc M. Mareel
    Pages 93-106
  6. Gero Wedemann, Anja Bethge, Volker Haustein, Udo Schumacher
    Pages 107-116
  7. Raffaella Giavazzi, Alessandra Decio
    Pages 131-140
  8. Michael Didié, Wolfram-Hubertus Zimmermann
    Pages 181-190
  9. Rosemary Jeffery, Pooja Seedhar, Richard Poulsom
    Pages 235-245
  10. Deepashree Bapu, Munira Khadim, Susan A. Brooks
    Pages E1-E1

About this book

Introduction

The second edition of Metastasis Research Protocols brings together updated versions of the seminal technique that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important area of biology. Volume 2 presents techniques applicable at the level of living cells and tissues, and presents methodologies applicable to cell behaviour in vitro, in animal models and in mathematical constructs. The aim is the study of  the interaction between cancer cells and their host/environment. The focus throughout is on the tools that have been shown to be helpful in unravelling the processes important in cancer metastasis.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Metastasis Research Protocols, Second Edition: Volume 2 seeks to aid scientists in the further study of new methods in the area of metastasis research.

Keywords

PCR RNAi technology SDS-PAGE affinity measurement of biomolecular interactions development of metastases in vivo endothelial cells extracellular matrix components fluorescent imaging techniques immunochemistry metastasis research methylation analysis of microRNA syngeneic and xenograft animal models vivo double knock-out models

Editors and affiliations

  • Miriam Dwek
    • 1
  • Udo Schumacher
    • 2
  • Susan A. Brooks
    • 3
  1. 1.Molecular and Applied BiosciencesUniversity of Westminster School of Life SciencesLondonUnited Kingdom
  2. 2.MorphologieUniversitätsklinikum Hamburg Inst. Anatomie und ExperimentelleHamburgGermany
  3. 3.Oxford Brookes University School of Life SciencesOxfordUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4614-8244-4
  • Copyright Information Springer Science+Business Media, New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4614-8243-7
  • Online ISBN 978-1-4614-8244-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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