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  • © 2013

Laboratory Protocols in Fungal Biology

Current Methods in Fungal Biology

  • Consolidates the most widely used mycological methods available in the fields of biochemistry, biotechnology and microbiology
  • Features the work of the foremost authorities on fungal biology
  • Multi-purpose book for a diverse audience
  • Includes supplementary material: sn.pub/extras

Part of the book series: Fungal Biology (FUNGBIO)

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Table of contents (57 chapters)

  1. Protocol for the In Vivo Quantification of Superoxide Radical in Fungi

    • Konstantinos Grintzalis, Ioannis Papapostolou, Christos Georgiou
    Pages 259-264
  2. Isolation of Intact RNA from Sorted S. cerevisiae Cells for Differential Gene Expression Analysis

    • Jeannette Vogt, Frank Stahl, Thomas Scheper, Susann Müller
    Pages 265-277
  3. Quantitative PCR Analysis of Double-Stranded RNA-Mediated Gene Silencing in Fungi

    • José J. de Vega-Bartol, Vega Tello, Jonathan Niño, Virginia Casado, José M. Díaz-Mínguez
    Pages 279-287
  4. Proteomic Protocols for the Study of Filamentous Fungi

    • Raquel González Fernández, Jesús V. Jorrín Novo
    Pages 299-308
  5. Real-Time PCR Assay in Fungi

    • Naomichi Yamamoto
    Pages 331-336
  6. Quantitative Sampling Methods for the Analysis of Fungi: Air Sampling

    • Mary C. O’Loughlin, Katherine D. Turner, Kevin M. Turner
    Pages 337-341
  7. Transformation of Filamentous Fungi in Microtiter Plate

    • Bianca Gielesen, Marco van den Berg
    Pages 343-347
  8. Molecular Fingerprinting of Fungal Communities in Soil

    • Roberto A. Geremia, Lucie Zinger
    Pages 349-356
  9. Rapid Deletion Plasmid Construction Methods for Protoplast and Agrobacterium-based Fungal Transformation Systems

    • María D. García-Pedrajas, Zahi Paz, David L. Andrews, Lourdes Baeza-Montañez, Scott E. Gold
    Pages 375-393
  10. Improved Transformation Method for Alternaria Brassicicola and Its Applications

    • Yangrae Cho, Akhil Srivastava, Christopher Nguyen
    Pages 395-401
  11. Methods for High-Quality DNA Extraction from Fungi

    • Vijai Kumar Gupta, Maria G. Tuohy, Rajeeva Gaur
    Pages 403-406
  12. Nucleic and Protein Extraction Methods for Fungal Exopolysaccharide Producers

    • Jochen Schmid, Dirk Mueller-Hagen, Volker Sieber, Vera Meyer
    Pages 427-434

About this book

Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enablesthe reader to progress from elementary concepts to advanced research techniques.  Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals.

Coming Soon in the Fungal Biology series:

 

Goyal, Manoharachary / Future Challenges in Crop Protection Against Fungal Pathogens

Martín, García-Estrada, Zeilinger / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites

Zeilinger, Martín, García-Estrada / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites, Volume 2

van den Berg, Maruthachalam / Genetic Transformation Systems in Fungi

Schmoll, Dattenbock / Gene Expression Systems in Fungi

Dahms / Advanced Microscopy in Mycology

Reviews

From the book reviews:

“The book ‘Laboratory Protocols in Fungal Biology’ written by international researchers, offers diverse modern laboratory techniques in the field of fungal biology. … Each chapter is comprehensive, provides readily reproducible protocols and is easy to grasp by beginners. This book is a valuable guide to budding mycologists as well as professionals. It is recommended to all the Universities. A crucial Laboratory Manual for Fungal Biologists, Taxonomists, Fungal Biotechnologists, Biochemists, Histochemists, Plant Pathologists and Laboratory technicians.” (Melvina D’souza and Samantha C. Karunarathna, Fungal Diversity, December, 2014)

“This new book is very much hands-on, and covers an extraordinarily diverse range of topics. … Chapters are well-referenced to primary literature, and there are numerous links to website. There are numerous illustrations, most clearly presented, and in some cases using colour. This is a reference work that deserves to be accessible in all mycology laboratories … .” (IMA Fungus, Vol. 4 (1), 2013)

Editors and Affiliations

  • , Department of Biochemistry, National University of Ireland Galway, Galway, Ireland

    Vijai Kumar Gupta, Maria G. Tuohy, Manimaran Ayyachamy

  • , Manufacturing Sciences and Technology, Pfizer Ireland Pharmaceuticals, Clondalkin, Ireland

    Kevin M. Turner

  • , School of Natural Sciences, National University of Ireland Galway, Galway, Ireland

    Anthonia O’Donovan

About the editors

Editors

Vijai Kumar Gupta is Assistant Professor of Biotechnology at MITS University, India. Presently, he is working as a Post Doctoral Research Scientist, at NUIG, Ireland. He obtained his Ph.D. in Microbiology from Dr. R.M.L. Avadh University, India. He has been honoured with several awards, including the prestigious Indian ICAR Senior Research Fellowship and Young Scientist Award-2009, 2011. He has over seven years of research experience in Applied Microbiology and Biotechnology with a special interest in fungal biology.  He has edited books published from Lambert Academic Publishing, Germany; Nova Science Publishers, USA; Science Publisher, CRC Press, Taylor & Francis, USA; and Springer, USA.

Dr. Maria G. Tuohy is the Head of the Molecular Glycobiotechnology Research Group, NUI Galway. She has more than 20 years experience in the molecular biochemistry, genetics and biotechnology of fungi, with a special interest in thermophilic ascomycetes and the characterization of these fungi as cell factories for protein production, including novel thermostable enzymes/enzyme systems. Dr. Tuohy has authored 132 research publications. She is also a reviewer for international journals and funding agencies, an associate editor for Microbiology and co-editor of four books with Dr. V. K. Gupta.

Associate Editors

Dr. Manimaran Ayyachamy is a Research Scientist with NUIG, Ireland. He obtained his PhD in Microbiology from the University of Madras, India. He has twelve years of research experience in microbial biotechnology with a special interest in microbial enzymes and biofuels technology. He has co-edited books published from Lambert Academic Publishing, Germany; Nova Science Publishers, USA; Science Publisher, CRC Press, Taylor & Francis, USA; and Springer, USA with Dr. V.K. Gupta

Dr. Kevin M. Turner is a Research Scientist in the division of Manufacturing Sciences and Technology in Pfizer Ireland Pharmaceuticals, Dublin. He obtained his PhD from the University of Limerick, Ireland. He has three years of research experience in microbial biotechnology.

Dr. Anthonia O’Donovan is a Postdoctoral Research Scientist with NUIG, Ireland. She obtained her PhD in Biochemistry from the National University of Ireland Galway, Ireland. She has seven years of research experience in microbial enzymes and biofuels technology.

Bibliographic Information

Buy it now

Buying options

eBook USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 249.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access