Molecular Biology of Erythropoiesis

1. Front Matter

   Pages i-xiii

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2. Biology of Erythropoietin

   1. Erythropoietin: From Mountain Top to Bedside

      • A. J. Erslev, J. Caro

      Pages 1-7

   2. Serum Erythropoietin and Hemoglobin Affinity for Oxygen in Patients Phlebotomized for Polycythemia Vera

      • Ruth A. Cohen, Gisela Clemons, Shirley Ebbe

      Pages 9-15

   3. Erythropoietin and the Polycythemia of High-Altitude Dwellers

      • Nicholas Dainiak, Hilde Spielvogel, Sandra Sorba, Leon Cudkowicz

      Pages 17-21

   4. Enhanced Effect Of Increased Erythrocyte Production Rate On Plasma Erythropoietin Levels Of Mice During Subsequent Exposure To Hypobaria

      • Carlos E. Bozzini, Rosa M. Alippi, Ana C. Barcelo

      Pages 23-27

   5. Metabolism of Recombinant Human Erythropoietin in the Rat

      • Jerry L. Spivak

      Pages 29-38

   6. Regulation of Extrarenal Erythropoietin Production

      • Walter Fried

      Pages 39-51

   7. Structure and Role of Carbohydrate in Human Erythropoietin

      • Minoru Fukuda, Hiroshi Sasaki, Michiko N. Fukuda

      Pages 53-67

   8. Cellular Localization of Erythropoietin Gene Transcription

      • Catherine Lacombe, Jean-Louis Da Silva, Patrick Bruneval, Jean-Loup Salzmann, Nicole Casadevall, Jean-Pierre Camilleri et al.

      Pages 69-74

   9. Regulation of the Erythropoietin Gene

      • Shigehiko Imagawa, Mark A. Goldberg, H. Franklin Bunn

      Pages 75-85

3. Heme Regulation and Erythropoiesis

   1. Studies of the Effect of Erythropoietin on Heme Synthesis

      • Nega Beru, Eugene Goldwasser

      Pages 87-94

   2. Effects of Hemin on Erythropoiesis

      • Blanche P. Alter, J. Matthew Schofield, Liya He, Rona S. Weinberg

      Pages 95-102

   3. The Effect of Dimethyl Sulfoxide on Heme Synthesis and the Acute Phase Reaction in Human HepG2 Hepatoma Cells

      • Shigeru Sassa, Fuyuki Iwasa, Richard Galbraith

      Pages 103-114

   4. Cytochrome P450 Dependent Arachidonic Acid Metabolism in Hemopoietic Cells

      • J. D. Lutton, M. Laniado Schwartzman, N. G. Abraham

      Pages 115-121

   5. Regulation of Heme Biosynthesis in Chick Embryo Liver Cells

      • G. S. Marks, J. E. Mackie, S. A. McCluskey, D. S. Riddick

      Pages 123-133

4. Globin Gene Expression

   1. Long-Term Expression of the Human ß-Globin Gene After Retroviral Transfer into Pluripotent Hematopoietic Stem Cells of the Mouse

      • Richard E. Gelinas, Michael A. Bender, A. Dusty Miller, Ulrike Novak

      Pages 135-148

   2. Retroviral Gene Transfer in Mice: The Use of a Unique Packaging Line Improves Efficiency

      • Charles S. Hesdorffer, Dina Markowitz, Maureen Ward, Norma B. Lerner, Arthur Bank

      Pages 149-160

   3. Expression of the Globin Genes and Hematopoiesis in Beta-Thalassemic Mice

      • Raymond A. Popp, Susan L. Bolch, Sarah G. Shinpock, Diana M. Popp

      Pages 161-176

   4. Butyric Acid Modulates Developmental Globin Gene Switching in Man and Sheep

      • Susan P. Perrine, Paul Swerdlow, Douglas V. Faller, Gene Qin, Abraham M. Rudolph, James Reczek et al.

      Pages 177-183

5. Erythropoiesis — Regulatory Aspects

   1. Tumor Necrosis Factor α and the Anemia of Chronic Disease: Effects of Chronic Exposure to TNF on Erythropoiesis in Vivo

      • G. D. Roodman, R. A. Johnson, U. Clibon

      Pages 185-196

This volume presents the proceedings of the Fourth Annual Symposium on the Molecular Biology of Hemopoiesis, held in Reno, Nevada, November 1 and 2, 1988. Its focus on erythropoiesis represents an attempt to cover a rapidly expanding field, which has gone from elegant studies of erythro­ poietin physiology, to molecular biology, to clinical applications and again to physiology. The rapid development has been made possible by cloning of erythropoietin gene and the availability of recombinant hormone. The regulation of heme and its derivatives has also been aided by techniques of molecular biology; there is now a concerted effort to better understand how these enzymes contribute to proliferation, differentiation and maturation of the erythron. Globin gene derrangements have been targets of recent research in an attempt to correct the defect by genetic engineering. In the chapters of this book, several groups "expressed" their views on this subject. Finally, we analyze various regulators of erythropoiesis, both in vivo and in vitro. Dr. Richard Levere was a pioneer in many studies of heme metabolism and of erythropoiesis. He has been a generous supporter of research in this field and of our past meetings. It is only. fitting that this volume should be dedicated to him.

• Hydra
• enzymes
• erythrocyte
• gene expression
• metabolism
• physiology
• regulation
• transcription

• University of Connecticut Health Center, Farmington, USA

  Joao L. Ascensao

• Veterans Administration Medical Center, Reno, USA

  Esmail D. Zanjani

• Veterans Administration Hospital, Jackson, USA

  Mehdi Tavassoli

• National Heart, Lung, and Blood Institute, Bethesda, USA

  Alan S. Levine

• University of Nevada, Reno, USA

  F. Roy MacKintosh

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