Neuroanatomical Techniques

1. Front Matter

   Pages i-16

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2. The Methylene Blue Technique: Classic and Recent Applications to the Insect Nervous System

   • S. I. Plotnikova, G. A. Nevmyvaka

   Pages 1-14

3. Permanent Staining of Tracheae with Trypan Blue

   • G. E. Gregory

   Pages 15-19

4. Toluidine Blue as a Rapid Stain for Nerve Cell Bodies in Intact Ganglia

   • J. S. Altman

   Pages 21-24

5. Demonstration of Neurosecretory Cells in the Insect Central Nervous System

   • A. A. Panov

   Pages 25-50

6. Histochemical Demonstration of Biogenic Monoamines (Falck-Hillarp Method) in the Insect Nervous System

   • Nikolai Klemm

   Pages 51-73

7. The Bodian Protargol Technique

   • G. E. Gregory

   Pages 75-95

8. Reduced Silver Impregnations Derived from the Holmes Technique

   • A. D. Blest, P. S. Davie

   Pages 97-118

9. Reduced Silver Impregnations of the Ungewitter Type

   • A. D. Blest

   Pages 119-130

10. The Golgi Method: Its Application to the Insect Nervous System and the Phenomenon of Stochastic Impregnation

    • N. J. Strausfeld

    Pages 131-203

11. Electron-Microscopic Methods for Nervous Tissues

    • M. P. Osborne

    Pages 205-239

12. Methods for Special Staining of Synaptic Sites

    • Friedrich-Wilhelm Schürmann

    Pages 241-261

13. Experimental Anterograde Degeneration of Nerve Fibers: A Tool for Combined Light- and Electron-Microscopic Studies of the Insect Nervous System

    • Friedrich-Wilhelm Schürmann

    Pages 263-281

14. Simple Axonal Filling of Neurons with Procion Yellow

    • G. E. Gregory

    Pages 283-290

15. Intracellular Staining with Fluorescent Dyes

    • L. G. Bishop

    Pages 291-306

16. Intracellular Staining of Insect Neurons with Procion Yellow

    • Roland Hengstenberg, Bärbel Hengstenberg

    Pages 307-324

17. The Use of Horseradish Peroxidase as a Neuronal Marker in the Arthropod Central Nervous System

    • Hendrik E. Eckert, C. Bruce Boschek

    Pages 325-339

18. Cobalt Staining of Neurons by Microelectrodes

    • Michael O’Shea

    Pages 341-355

19. Nonrandom Resolution of Neuron Arrangements

    • J. Bacon, N. J. Strausfeld

    Pages 357-372

20. Filling Selected Neurons with Cobalt through Cut Axons

    • J. S. Altman, N. M. Tyrer

    Pages 373-402

Most neurobiological research is performed on vertebrates, and it is only natural that most texts describing neuroanatomical methods refer almost exclusively to this Phylum. Nevertheless, in recent years insects have been studied intensively and are becoming even more popular in some areas of research. They have advantages over vertebrates with respect to studying genetics of neuronal development and with respect to studying many aspects of integration by uniquely identifiable nerve cells. Insect central nervous system is characterized by its compactness and the rather large number of nerve cells in a structure so small. But despite their size, parts of the insect eNS bear structural comparisons with parts of vertebrate eNS. This applies particularly to the organization of the thoracic ganglia (and spinal cord), to the insect and vertebrate visual sys­ tems and, possibly, to parts of the olfactory neuropils. The neurons that make up these areas in insects are often large enough to be impaled by microelectrodes and can be injected with dyes. Added to advantages of using a small eNS, into which the sensory periphery is precisely mapped, are the many aspects of insect behaviour whose components can be quan­ titized and which may find both structural and functional correlates within clearly defined regions of neuropil. Together, these various features make the insect eNS a rewarding object for study. This volume is the first of two that describe both classic and recent methods for neuroanatomical research on insect eNS.

• Insekten
• Nervensystem
• Neuroanatomy
• development
• insect
• nervous system
• tissue

• European Molecular Biology Laboratory, Heidelberg, Germany

  N. J. Strausfeld

• Department of Entomology, University of California, Riverside, USA

  Thomas A. Miller

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