Methods in Protein Sequence Analysis

1. Coordination of Protein and DNA Sequencing

   1. Correlation Between DNA and Protein Sequences in β-Galactosidase Related Proteins

      • Audree V. Fowler, Irving Zabin

      Pages 363-371

   2. The Molecular Consequences of Formaldehyde and Ethyl Methanesulfonate Mutagenesis in Drosophila: Analysis of Mutants in the Alcohol Dehydrogenase Gene

      • Allen R. Place, Cheeptip Benyajati, William Sofer

      Pages 373-379

2. Use of High Performance Liquid Chromatrography (HPLC) for the Separation of Proteins, Peptides, and Amino Acids

   1. Front Matter

      Pages 381-381

      PDF

   2. Protein Microsequencing by HPLC Peptide Purification and Solid Phase Edman Degradation

      • James J. L’Italien, Richard A. Laursen

      Pages 383-399

   3. Reverse Phase High-Performance Liquid Chromatography for Protein Purification: Insulin-Like Growth Factors, Ca2+- Binding Proteins and Metallothioneins

      • Kenneth J. Wilson, Martin W. Berchtold, Peter Zumstein, Stephan Klauser, Graham J. Hughes

      Pages 401-408

   4. Reversed Phase (RP) HPLC of Proteins and Peptides and Its Impact on Protein Microsequencing

      • Louis Henderson, Ray Sowder, Stephen Oroszlan

      Pages 409-416

   5. Preparative Isolation of Ia Antigen Membrane Protein Component Polypeptides on C18 Reverse Phase HPLC

      • D. J. McKean, M. Bell

      Pages 417-426

   6. Chromatography and Rechromatography in HPLC Separation of Peptides

      • H. Kratzin, C. Y. Yang, N. Hilschmann

      Pages 427-434

   7. Sequence Analysis of Membrane Protein Fragments Isolated by High Pressure Liquid Chromatography

      • Francis S. Heinemann, Juris Ozols

      Pages 435-446

   8. Preparation of Peptides and Proteins for Sequence Analysis at the Low Nanomole to Subnanomole Level by Reverse-Phase High-Performance Liquid Chromatography: Results for Cytochromes P450 and Fibronectin

      • John E. Shively, Hema Pande, Pau-Miau Yuan, David Hawke

      Pages 447-454

   9. Analytical and Preparative Applications of High-Performance Liquid Chromatography. Comparisons with Other Methods

      • Hedvig von Bahr-Lindström, Mats Carlquist, Viktor Mutt, Hans Jörnvall

      Pages 455-462

   10. Separation of Hinge Glycopeptides of Human IgD by HPLC

       • Nobuhiro Takahashi, Daniel Tetaert, Frank W. Putnam

       Pages 463-470

   11. Use of High Performance Liquid Chromatography in Characterizing Nucleotide Binding Sites and Antigenic Determinants in cAMP-Dependent Protein Kinase

       • Susan Taylor, Anthony Kerlavage, Norman Nelson, Sharon Weldon, Mark Zoller

       Pages 471-478

   12. The Use of Radially-Compressed Reversed Phase HPLC for the Separation of Tryptic Peptides of Apolipoproteins

       • W. A. Bradley, A. M. Gotto Jr., J. T. Sparrow

       Pages 479-488

   13. Routine Peptide Mapping by High Performance Liquid Chromatography

       • C. S. Fullmer, R. H. Wasserman

       Pages 489-497

   14. Comparative Peptide Mapping by HPLC: Identification of Single Amino Acid Substitutions in Temperature Sensitive Mutants

       • K. R. Williams, J. J. L’Italien, R. A. Guggenheimer, L. Sillerud, E. Spicer, J. Chase et al.

       Pages 499-507

   15. A New Low Cost, Fully Automated Amino Acid Analyzer Using a Gradient HPLC

       • David G. Klapper

       Pages 509-515

3. Identification of Phenylthiohydantoins

   1. Front Matter

      Pages 517-517

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   2. Fused Silica-Capillary-Gas Chromatography. A Novel Procedure for the Identification of PTH in the Analysis of Protein

      • D. Tripier

      Pages 519-527

4. Communications: Sequence Methods

   1. Front Matter

      Pages 529-529

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Methods in Protein Sequence Analysis contains an intensely prac­ tical account of all the new methodology available to scientists carrying out protein and peptide sequencing studies. Many of the striking advances in fields as diverse as immunology, cell motility, and neurochemistry have in fact been fueled by our ever more powerful ability to determine the sequences and structures of key proteins and peptides. It is our hope that the rich array of tech­ niques and methods for sequencing proteins discussed in this volume-methods that generate much of the information crucial to progress in modern biology-will now become accessible to all who can benefit from them. The papers of the present volume constitute the Proceedings of the IVth International Conference on Methods in Protein Se­ quence Analysis, which was held at Brookhaven National Labo­ ratory, Upton, NY, September 21-25, 1981. It was the most recent in a series of biennial conferences, the previous one having been held in Heidelberg, GFR, in 1979. The series was originated by Richard Laursen, and initially dealt with one aspect of the field, solid-phase sequencing. The scope of the meeting was very broad and among the many aspects of protein sequencing discussed were: instrumentation, strategy, chemicals, mass spectrometry, cleavage of proteins and separation of peptides, and solid, liquid, manual, and even "gas­ phase" sequencing.

• ATP
• ATP-Synthase
• Amino acid
• DNA
• Methodologie
• Nucleotide
• RNA
• enzymes
• primary structure
• protein sequence
• protein structure
• proteins

• Brookhaven National Laboratory, Upton, USA

  Marshall Elzinga

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