Methods in Protein Sequence Analysis

1. Solid Phase Sequencing

   1. The use of Dabitc in Automated Solid-Phase Sequencing

      • Johann Salnikow, Arnold Lehmann, Brigitte Wittmann-Liebold

      Pages 181-188

   2. Improved Automatic Conversion for Use with a Liquid-Phase Sequenator

      • Michael N. Margolies, Andrew Brauer, Christine Oman, David G. Klapper, Marcus J. Horn

      Pages 189-203

   3. A Study of the Edman Degradation in the Assessment of the Purity of Synthetic Peptides

      • Stephen B. H. Kent, Mark Riemen, Marie LeDoux, R. B. Merrifield

      Pages 205-213

   4. Solid Phase Synthesis of Polypeptides and Oligonucleotides Using a Solid Phase Sequencer

      • Alex G. Bonner, Marcus J. Horn, Richard S. Neves, Stephen B. H. Kent

      Pages 215-219

2. Manual Sequencing

   1. Front Matter

      Pages 221-221

      PDF

   2. Manual Batchwise Sequencing Methods

      • George E. Tarr

      Pages 223-232

   3. Micro Quantitative Edman Manual Sequencing

      • Clyde Zalut

      Pages 233-239

3. Sequencing by Mass Spectrometry

   1. Front Matter

      Pages 241-241

      PDF

   2. Protein Sequencing by Mass Spectrometry

      • H. R. Morris, G. W. Taylor, M. Panico, A. Dell, A. T. Etienne, R. A. McDowell et al.

      Pages 243-261

   3. Sequence Analysis of Polypeptides by Direct Chemical Ionization Mass Spectrometry

      • Steven A. Carr, Vernon N. Reinhold

      Pages 263-270

   4. A New Computer-Aided Method for Sequencing a Polypeptide from the Masses and Edman-Degradation of its Constituent Peptide Fragments

      • Yasutsugu Shimonishi

      Pages 271-278

   5. The Complementarity of Mass Spectrometry to Edman Degradation or DNA Based Protein Sequencing

      • K. Biemann

      Pages 279-288

4. Cleavage of Proteins

   1. Front Matter

      Pages 289-289

      PDF

   2. Enzymic Cleavage of Proteins

      • B. Keil

      Pages 291-304

   3. Unusual Enzymatic Cleavage and Preparative Peptide Separation by High Pressure Liquid Chromatography in the Establishment of the Amino Acid Sequence of Tubulin

      • Herwig Ponstingl, Erika Krauhs, Melvyn Little, Wolfgang Ade, Roland Weber

      Pages 305-312

   4. Chemical Cleavage of Proteins

      • Mark A. Hermodson

      Pages 313-323

   5. Protein Fragmentation with o-Iodosobenzoic Acid

      • A. Fontana, D. Dalzoppo, C. Grandi, M. Zambonin

      Pages 325-334

5. Coordination of Protein and DNA Sequencing

   1. Front Matter

      Pages 335-335

      PDF

   2. Analysis of the Escherichia Coli ATP-Synthase Complex by DNA and Protein Sequencing

      • John E. Walker, Alex Eberle, Nicholas J. Gay, Peter Hanisch, Matti Saraste, Michael J. Runswick

      Pages 337-354

   3. The Primary Structure of Escherichia Coli RNA Polymerase. Nucleotide Sequences of the rpoB and rpoC Genes and Amino Acid Sequences of the β and β’ Subunits

      • V. M. Lipkin, E. D. Sverdlov, G. S. Monastyrskaya, Yu. A. Ovchinnikov

      Pages 355-362

Methods in Protein Sequence Analysis contains an intensely prac­ tical account of all the new methodology available to scientists carrying out protein and peptide sequencing studies. Many of the striking advances in fields as diverse as immunology, cell motility, and neurochemistry have in fact been fueled by our ever more powerful ability to determine the sequences and structures of key proteins and peptides. It is our hope that the rich array of tech­ niques and methods for sequencing proteins discussed in this volume-methods that generate much of the information crucial to progress in modern biology-will now become accessible to all who can benefit from them. The papers of the present volume constitute the Proceedings of the IVth International Conference on Methods in Protein Se­ quence Analysis, which was held at Brookhaven National Labo­ ratory, Upton, NY, September 21-25, 1981. It was the most recent in a series of biennial conferences, the previous one having been held in Heidelberg, GFR, in 1979. The series was originated by Richard Laursen, and initially dealt with one aspect of the field, solid-phase sequencing. The scope of the meeting was very broad and among the many aspects of protein sequencing discussed were: instrumentation, strategy, chemicals, mass spectrometry, cleavage of proteins and separation of peptides, and solid, liquid, manual, and even "gas­ phase" sequencing.

• ATP
• ATP-Synthase
• Amino acid
• DNA
• Methodologie
• Nucleotide
• RNA
• enzymes
• primary structure
• protein sequence
• protein structure
• proteins

• Brookhaven National Laboratory, Upton, USA

  Marshall Elzinga

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