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  • © 2022

RNAi Strategies for Pest Management

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2360)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiv
  2. Guiding RNAi Design Through Characterization of Endogenous Small RNA Pathways

    • Jacob O. Peter, Yulica Santos-Ortega, Alex Flynt
    Pages 33-47
  3. RNAi Feeding Bioassay: A Protocol for dsRNA Screening Against Asian Citrus Psyllid and Related Hemipteran Insects

    • Jonatha dos Santos Silva, Layanna Rebouças de Santana Cerqueira, Wayne Bextine Hunter, Eduardo Chumbinho de Andrade
    Pages 85-90
  4. Functional Characterization of Target Genes Associated with Insecticide Resistance of the Green Peach Aphid, Myzus persicae

    • John Fosu-Nyarko, Sadia Iqbal, Frances Brigg, Michael G. K. Jones
    Pages 187-208
  5. RNAi Expression in Cotton for Control of Herbivorous Insects

    • Ricardo Salvador, Laura Maskin, José Niz, Mariana Turica, Analía Pedarros, Esteban Hopp et al.
    Pages 217-233
  6. Transplastomic Tomato Plants Expressing Insect-Specific Double-Stranded RNAs: A Protocol Based on Biolistic Transformation

    • Emine Kaplanoglu, Igor Kolotilin, Rima Menassa, Cam Donly
    Pages 235-252Open Access
  7. Double-Strand RNA (dsRNA) Delivery Methods in Insects: Diaphorina citri

    • Yulica Santos-Ortega, Alex Flynt
    Pages 253-277

About this book

This volume explores the latest techniques used for sustainable development of eco-friendly pest management systems. Chapters in this book look at the design of novel RNAi-mediated pest control strategies and related issues. Some of the topics covered in this book are identification and characterization of differentially expressed non-coding RNAs in insect genomes; functional small RNA design; analysis of endogenous RNAi pathways; RNAi screening; plant models of RNAi-mediated pest control; and dsRNA delivery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Cutting-edge and comprehensive, RNAi Strategies for Pest Management: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this exciting and developing field. 


Chapter 18 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.



Editors and Affiliations

  • Oncativo, Argentina

    Luis María Vaschetto

Bibliographic Information

  • Book Title: RNAi Strategies for Pest Management

  • Book Subtitle: Methods and Protocols

  • Editors: Luis María Vaschetto

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1633-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1632-1Published: 09 September 2021

  • Softcover ISBN: 978-1-0716-1635-2Published: 10 September 2022

  • eBook ISBN: 978-1-0716-1633-8Published: 08 September 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 369

  • Number of Illustrations: 14 b/w illustrations, 49 illustrations in colour

  • Topics: Genetics and Genomics, Agriculture

  • Industry Sectors: Biotechnology

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access