© 2022

DNA Modification Detection Methods

  • Bi-Feng Yuan
  • Provides a comprehensive overview of available techniques and methods for studying various types of DNA modifications

  • Organized as detailed step-by-step protocols for experimental procedures required to successfully perform analysis

  • Offers new opportunities for researchers to manipulate the modification status to affect gene expression, and develop small molecules to tune the DNA functions


Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-vi
  2. Detection of 5mC, 5hmC, 5fC, and 5caC

    1. Front Matter
      Pages 1-1
    2. Yael Michaeli, Sapir Margalit, Sigal Avraham, Hila Erez, Noa Gilat, Zuzana Tulpová et al.
      Pages 31-44
    3. Lin Cui, Juan Hu, Meng Wang, Chen-Chen Li, Chun-Yang Zhang
      Pages 45-52
    4. Zong Dai, Li Zhang, Si-Yang Liu, Yuzhi Xu, Danping Chen, Jun Chen et al.
      Pages 53-63
    5. Natsumi Taka, Wataru Yoshida
      Pages 93-102
  3. Detection of 6mA

  4. Detection of 5hmU and 5fU

  5. Detection of Base J and 8-oxo-7,8-dihydroguanine

    1. Front Matter
      Pages 163-163

About this book


Understanding the functional roles of DNA modifications relies on the accurate detection, quantification, and mapping of DNA modifications. Methods for deciphering DNA modifications have substantially improved over the last several years, which greatly revolutionize the field of DNA modifications. In addition to DNA cytosine methylation (5-methylcytosine, 5mC), the best-characterized epigenetic modification, many new modifications have been discovered to present in DNA in recent years.  

This book provides a comprehensive overview of available techniques and methods together with detailed step-by-step protocols for experimental procedures required to successfully perform analysis on various types of DNA modifications, including 5mC, 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), 5-carboxycytosine (5caC), 5-hydroxymethyluracil (5hmU), 5-formyluracil (5fU), N6-methyladenine (6mA), β-D-glucosyl-5-hydroxymethyluracil (base J) and 8-oxo-7,8-dihydroguanine (OG). This laboratory manual is a valuable source for biochemists and molecular biologists from different fields who wish to investigate DNA modifications.


DNA modification Detection Mapping Mass spectrometry DNA Sequencing

Editors and affiliations

  • Bi-Feng Yuan
    • 1
  1. 1.Department of ChemistryWuhan UniversityWuhanChina

About the editors

Dr. Yuan received his bachelor’s degree and PhD degree from Wuhan University in 2001 and 2006, respectively. After completing the postdoctoral research in National University of Singapore (2006-2007, Singapore) and University of California, Riverside (2007-2010, USA), Dr. Yuan started to work as a professor in the College of Chemistry and Molecular Sciences of Wuhan University from 2011. Dr. Yuan’s research focuses on the development and application of new analytical techniques in the investigation of the occurrence, location, and biological functions of nucleic acid modifications. By establishing highly sensitive analytical methods, Dr. Yuan discovered many new types of modifications in nucleic acids. He also revealed the mechanisms of the formation and metabolism of some nucleic acid modifications, which promotes the understanding of the biological functions of nucleic acid modifications.

Bibliographic information

  • Book Title DNA Modification Detection Methods
  • Editors Bi-Feng Yuan
  • Series Title Springer Protocols Handbooks
  • Series Abbreviated Title Springer Protocols Handbooks
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2022
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-0716-1228-6
  • Softcover ISBN 978-1-0716-1231-6
  • eBook ISBN 978-1-0716-1229-3
  • Series ISSN 1949-2448
  • Series E-ISSN 1949-2456
  • Edition Number 1
  • Number of Pages VI, 194
  • Number of Illustrations 18 b/w illustrations, 30 illustrations in colour
  • Topics Biomedical Engineering/Biotechnology
    Gene Expression
    Biological Techniques
  • Buy this book on publisher's site
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