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Pollen and Pollen Tube Biology

Methods and Protocols

  • Anja Geitmann
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2160)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Hiroyuki Kakui, Misako Yamazaki, Naoto-Benjamin Hamaya, Kentaro K. Shimizu
    Pages 1-11
  3. Said Hafidh, David Honys
    Pages 41-72
  4. Taro Takahashi, Toshiyuki Mori, Tomoko Igawa
    Pages 73-81
  5. Sheng Zhong, Zhijuan Wang, Li-Jia Qu
    Pages 83-92
  6. Jennifer A. Noble, Ravishankar Palanivelu
    Pages 109-128
  7. Mario Costa, Jessy Silva, Silvia Coimbra
    Pages 149-165
  8. Sheng Zhong, Zhijuan Wang, Li-Jia Qu
    Pages 181-190
  9. Daniel S. C. Damineli, Maria Teresa Portes, José A. Feijó
    Pages 201-210
  10. Hana Bertrand-Rakusová, Youssef Chebli, Anja Geitmann
    Pages 211-221
  11. Ana R. Sede, Diego L. Wengier, Cecilia Borassi, José M. Estevez, Jorge P. Muschietti
    Pages 233-242
  12. Jan T. Burri, Gautam Munglani, Bradley J. Nelson, Ueli Grossniklaus, Hannes Vogler
    Pages 275-292
  13. Back Matter
    Pages 327-332

About this book

Introduction

This volume explores a collection of experimental techniques used to investigate different aspects of pollen development and function, including its role in reaching the ovule and delivering the two sperm cells. The techniques discussed range from basic methodology to cultivate pollen in vitro to the sophisticated experiments involving micromanipulation, Lab-on-Chip technology, or high-end imaging. The chapters in this book cover topics such as pollen grain counting using a cell counter; restricted pollination for tracing individual pollen tubes in a pistil; obtaining mutant pollen for phenotypic analysis and pollen tube dual-staining; analyzing intracellular gradients in pollen tubes; and measuring exocytosis rate in Arabidopsis pollen tube using corrected fluorescence recovery after photoconversion (cFRAPc) technique. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Pollen and Pollen Tube Biology: Methods and Protocols is a valuable resource for novice and expert scientists interested in learning more about the field of plant reproduction. 

Keywords

gametophyte Bimolecular fluorescence Genome-wide association study (GWAS) RStudio RNA-Seq expression

Editors and affiliations

  • Anja Geitmann
    • 1
  1. 1.Department of Plant ScienceMcGill UniversityMontrealCanada

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-0716-0672-8
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0671-1
  • Online ISBN 978-1-0716-0672-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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