Experimental Protocols in Biotechnology

1. Front Matter

   Pages i-xv

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2. Enzyme-Linked Immunosorbent Assay Detection of Bacterial Wilt–Causing Ralstonia solanacearum

   • Pramila Devi Umrao, Vineet Kumar, Shilpa Deshpande Kaistha

   Pages 1-18

3. Diagnosis of Tropical Fascioliasis Using Specific Antigen Detection in Feces of Infected Human Being or Animal Species

   • Neelima Gupta, Said I. Shalaby, Dileep K. Gupta, Mona A. Awad

   Pages 19-27

4. CMV Promoter-Driven Expression and Visualization of Tagged Proteins in Live and Fixed Zebrafish Embryonic Epidermis

   • Kirti Gupta, Mahendra Sonawane

   Pages 29-41

5. Detection of Blood Parasites and Estimation of Hematological Indices in Fish

   • Neelima Gupta, Shahla Nigar

   Pages 43-73

6. Techniques to Conduct Morphological and Molecular Investigations on Nematodes

   • Aasha Rana, Anshika Yadav, Aashaq Hussain Bhat, Ashok Kumar Chaubey, Sandeep K. Malhotra

   Pages 75-94

7. Computational Approaches in Drug Designing and Their Applications

   • Dev Bukhsh Singh, Rajesh Kumar Pathak

   Pages 95-117

8. Bacteriophage Control for Pseudomonas aeruginosa Biofilm Formation and Eradication

   • Pramila Devi Umrao, Vineet Kumar, Sadhana Singh Sagar, Shilpa Deshpande Kaistha

   Pages 119-137

9. Detection of Phosphoproteins (Phosphoserine and Phosphothreonine) from Thylakoid Membranes Using Western Blotting

   • Varsha Gupta, Baishnab Charan Tripathy

   Pages 139-154

10. Estimation of Plasma Membrane and Tonoplast ATPase Activity in Plant Tissues

    • Neerja Srivastava

    Pages 155-162

11. Quantification of Conserved MicroRNA in Plants and Validation of New Targets

    • Lata Israni Shukla

    Pages 163-181

12. Isolation of Natural Killer Cells from Mouse Bone Marrow and Spleen

    • Asmita Das

    Pages 183-192

13. Gene Expression Analysis from Human Peripheral Blood Mononuclear Cells

    • Sipahee Lal Patel, Dinesh Kumar, Anamika Dwivedi, Payal Singh Raghuvanshi, Vishal Chand, Jaya Prakash et al.

    Pages 193-207

14. Use of Droplet PCR in Biomedical Research

    • Kyle A. Doxtater, Manish K. Tripathi, Murali M. Yallapu, Meena Jaggi, Subhash C. Chauhan

    Pages 209-214

15. Determination of Michaelis–Menten Enzyme Kinetics Parameters of Alkaline Phosphatase in Clinical Samples

    • Varsha Gupta, Abhishek Gupta, Lata I. Shukla, Abhimanyu Kumar Jha, Jaya Prakash, Baishnab Charan Tripathy

    Pages 215-236

16. A Novel Technique for the Detection of LncRNAs on Tissue Sections

    • Andrew E. Massey, Manish K. Tripathi, Murali M. Yallapu, Meena Jaggi, Subhash C. Chauhan

    Pages 237-243

17. Back Matter

    Pages 245-246

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This manual provides insights into the theory and practical aspects of several biotechnological and biochemical techniques for plants, protozoa, nematodes, insects and fishes, as well as human samples. The book also covers bioinformatics tools. The manual is an inclusive compilation, explaining techniques for microbial cultures, their diagnostics, DNA barcoding, microscopic techniques, blood analysis, parasite diagnostics through copro-antigens, enzyme analysis with enzyme kinetics, gene expression analysis, in-vivo protein visualization in live animals, geno-toxicity assays, quantification of micro RNAs and LncRNAs in tissue sections, the use of droplet PCR, and in-silico analysis. It provides step-by-step descriptions and details of each methodology, together with the final outcomes and inferences, in a simple and lucid manner, easily reproducible even for beginners. The broad range of techniques covered makes this volume extremely useful in understanding the principles of biotechniques, and simple applications for practical courses. 


               

• Quality assurance
• Sterilization
• Antibiotic sensitivity
• Quantitative PCR
• Cycle threshold

• Chhatrapati Shahu Ji Maharaj University, Kanpur, India

  Neelima Gupta

• Department of Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur, India

  Varsha Gupta

Prof. Neelima Gupta, M.Sc. Gold Medalist, M.Phil., Ph.D. in 1981 (Zoology) from the AMU, Aligarh, obtained her D.Sc. degree (Animal Science) from the MJP Rohilkhand University, Bareilly in 2012.  Presently working as Vice Chancellor of CSJM University, Kanpur, India since 2018.  Recipient of the Ek Janaki Ammal National Award for Animal Taxonomy (Ministry of Environment & Forests), Highest State Award, Saraswati Samman and Centre of Excellence (Government of UP), Vigyan Ratna Award (Council of Science & Technology, UP).  Has worked extensively on parasite taxonomy, aquatic toxicology of the Ramganga and Ganga Rivers, and fish health.  Nominated by the British Council, DST and INSA in various academic programs.

Dr. Varsha Gupta received her Ph.D (Life Sciences) from Jawaharlal Nehru University, New Delhi, in 2003. She then joined the National Centre for Plant Genome Research, New Delhi, for her post-doctoral training on plant genomics.  Presently working at Chhatrapati Shahu Ji Maharaj University, Kanpur since 2006.  She has done extensive work on molecular diagnostics using genomic and proteomic approaches, and has published several research articles and one textbook on “Basic and Applied Aspects of Biotechnology”.



              

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