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Single Molecule Microscopy in Neurobiology

  • Book
  • © 2020

Overview

Editors:
  1. Nobuhiko Yamamoto

    1. Laboratory of Cellular and Molecular Neurobiology, Graduate School of Frontier Biosciences, Osaka University, Suita, Japan

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    You can also search for this editor in PubMed   Google Scholar

  2. Yasushi Okada

    1. Laboratory for Cell Polarity Regulation, RIKEN Center for Biosystems Dynamics Research, Suita, Japan

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 154)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Single-Molecule Imaging of Intracellular Transport in Neurons and Non-neuronal Cells: From Microscope Optics to Sample Preparations

    • Jay Yuan Jian Wang, Taketoshi Kambara, Yasushi Okada
    Pages 1-10

  3. In Vivo Single-Molecule Tracking of Voltage-Gated Calcium Channels with Split-Fluorescent Proteins in CRISPR-Engineered C. elegans

    • Yunke Zhao, Fabien Pinaud
    Pages 11-37

  4. Nanocores and Liquid Droplets: Single-Molecule Microscopy of Neuronal Stress Granule Components

    • Benedikt Niewidok, Rainer Kurre, Roland Brandt
    Pages 39-57

  5. Live-Cell Single-Molecule Imaging with Optogenetics Reveals Dynamics of a Neuronal Activity-Dependent Transcription Factor

    • Hironobu Kitagawa, Noriyuki Sugo, Nobuhiko Yamamoto
    Pages 59-79

  6. Single-Molecule Imaging of Recycling Synaptic Vesicles in Live Neurons

    • Merja Joensuu, Ramon Martínez-Mármol, Mahdie Mollazade, Pranesh Padmanabhan, Frédéric A. Meunier
    Pages 81-114

  7. Approaching Protein-Protein Interactions in Membranes Using Single-Particle Tracking and Packing Coefficient Analysis

    • Marianne Renner, Antoine Triller
    Pages 115-129

  8. Synaptic Function and Neuropathological Disease Revealed by Quantum Dot-Single-Particle Tracking

    • Hiroko Bannai, Takafumi Inoue, Matsumi Hirose, Fumihiro Niwa, Katsuhiko Mikoshiba
    Pages 131-155

  9. Multipolarization Dark-Field Imaging of Single Endosomes in Microfluidic Neuronal Culture for Simultaneous Orientation and Displacement Tracking

    • Luke Kaplan, Bianxiao Cui
    Pages 157-171

  10. Practical Guidelines for Two-Color SMLM of Synaptic Proteins in Cultured Neurons

    • Xiaojuan Yang, Christian G. Specht
    Pages 173-202

  11. Single-Molecule Localization Microscopy Propelled by Small Organic Fluorophores with Blinking Properties

    • Akihiko Morozumi, Mako Kamiya, Yasuteru Urano
    Pages 203-227

  12. Highly Biocompatible Super-resolution Imaging: SPoD-OnSPAN

    • Tetsuichi Wazawa, Takashi Washio, Takeharu Nagai
    Pages 229-244

  13. Nanoscale Molecular Imaging of Presynaptic Active Zone Proteins in Cultured Hippocampal Neurons

    • Hirokazu Sakamoto, Shigeyuki Namiki, Kenzo Hirose
    Pages 245-259

  14. Three-Dimensional Super-Resolution Imaging of the Cytoskeleton in Hippocampal Neurons Using Selective Plane Illumination

    • Frances Camille M. Wu, Feby Wijaya Pratiwi, Chin-Yi Chen, Chieh-Han Lu, Wei-Chun Tang, Yen-Ting Liu et al.
    Pages 261-293

  15. A Protocol for Single-Molecule Translation Imaging in Xenopus Retinal Ganglion Cells

    • Florian Ströhl, Julie Qiaojin Lin, Francesca W. van Tartwijk, Hovy Ho-Wai Wong, Christine E. Holt, Clemens F. Kaminski
    Pages 295-308

  16. Investigating Molecular Diffusion Inside Small Neuronal Compartments with Two-Photon Fluorescence Correlation Spectroscopy

    • Kazuki Obashi, Shigeo Okabe
    Pages 309-328

  17. Back Matter

    Pages 329-331
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Keywords

About this book

This volume looks at the methodology and techniques used by experts to study how certain molecules function in specific locations, and their temporal patterns. Chapters in this book cover topics such as in vivo single-molecule tracking of voltage-gated calcium channels with split-fluorescent proteins in CRISPR-engineering C. elegans; protein-protein interactions in membranes using single particle tracking; neuropathological diseases revealed by quantum-dot single particle tracking; SPoD-OnSPAN; and investigating molecular diffusion inside small neuronal compartments with two-photon fluorescence correlation spectroscopy. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.


Cutting-edge and comprehensive, Single Molecule Microscopy is a valuable resource for any researcher interested in learning more about this important field. 


Editors and Affiliations

  • Laboratory of Cellular and Molecular Neurobiology, Graduate School of Frontier Biosciences, Osaka University, Suita, Japan

    Nobuhiko Yamamoto

  • Laboratory for Cell Polarity Regulation, RIKEN Center for Biosystems Dynamics Research, Suita, Japan

    Yasushi Okada

Bibliographic Information

  • Book Title: Single Molecule Microscopy in Neurobiology

  • Editors: Nobuhiko Yamamoto, Yasushi Okada

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-0716-0532-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0531-8Published: 29 April 2020

  • Softcover ISBN: 978-1-0716-0534-9Published: 29 April 2021

  • eBook ISBN: 978-1-0716-0532-5Published: 28 April 2020

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XIII, 331

  • Number of Illustrations: 21 b/w illustrations, 73 illustrations in colour

  • Topics: Neurosciences, Biological Microscopy

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Health & Hospitals, Pharma

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